Abstract:
:A cDNA coding for a new subunit of soluble guanylyl cyclase with a calculated molecular mass of 81.7 kDa was cloned and sequenced. On the basis of sequence homology, the new subunit appears to be an isoform of the alpha 1-subunit and was designated alpha 2 as the new subunit is very similar to the alpha 1-subunit in the middle and C-terminal part; it is quite diverse in the N-terminal part. Preceding experiments had shown that coexpression of the alpha 1- and beta 1-subunits is necessary to obtain a catalytically active guanylyl cyclase in COS cells [(1990) FEBS Lett. 272, 221-223]. The finding that the alpha 2-subunit was able to replace the alpha 1- but not the beta 1-subunit in expression experiments demonstrates the interchangeability of the alpha-subunit isoforms of soluble guanylyl cyclase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Harteneck C,Wedel B,Koesling D,Malkewitz J,Böhme E,Schultz Gdoi
10.1016/0014-5793(91)80871-ysubject
Has Abstractpub_date
1991-11-04 00:00:00pages
217-22issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80871-Yjournal_volume
292pub_type
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