Abstract:
:Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 cargo domain, rationally designed C-terminal truncations of KIF5A and KIF5C were generated and their co-association with TRAK1/2 determined by quantitative co-immunoprecipitations following co-expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Randall TS,Moores C,Stephenson FAdoi
10.1016/j.febslet.2013.09.049subject
Has Abstractpub_date
2013-11-29 00:00:00pages
3763-9issue
23eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00761-8journal_volume
587pub_type
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