Abstract:
:This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. The EGF-induced GRK2-EGFR complex formation was greatly reduced by perturbation of EGFR and Src tyrosine kinase activity. Furthermore, studies with GRK2 mutants showed that neither catalytic activity nor the N-terminal domain of GRK2 was required for EGF-induced GRK2-EGFR complex formation. However, overexpression of Gbetagamma scavengers blocked EGF-induced formation of GRK2-EGFR complex.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gao J,Li J,Chen Y,Ma Ldoi
10.1016/j.febslet.2004.11.057keywords:
subject
Has Abstractpub_date
2005-01-03 00:00:00pages
122-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01440-1journal_volume
579pub_type
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