Abstract:
:RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Armour KJ,Lehane DB,Pakdel F,Valotaire Y,Graham R,Russell RG,Henderson IWdoi
10.1016/s0014-5793(97)00680-7subject
Has Abstractpub_date
1997-07-07 00:00:00pages
145-8issue
1eissn
0014-5793issn
1873-3468pii
S0014579397006807journal_volume
411pub_type
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