Abstract:
:The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 microM (at pH 7.0); xylitol, 25 mM; NAD, 100 microM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Richard P,Toivari MH,Penttilä Mdoi
10.1016/s0014-5793(99)01016-9keywords:
subject
Has Abstractpub_date
1999-08-20 00:00:00pages
135-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01016-9journal_volume
457pub_type
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