Sequence effects of single base loops in intramolecular quadruplex DNA.

Abstract:

:We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Rachwal PA,Brown T,Fox KR

doi

10.1016/j.febslet.2007.03.040

subject

Has Abstract

pub_date

2007-04-17 00:00:00

pages

1657-60

issue

8

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(07)00308-0

journal_volume

581

pub_type

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