Abstract:
:A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by homologous recombination and subsequently analysed the DeltaSiR1 mutants. While DeltaSiR1 mutants showed no obvious alteration in sulfur metabolism, their regeneration from protoplasts and their ability to produce mature spores was significantly affected, highlighting an unexpected link between moss sulfate assimilation and development, that is yet to be characterized.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva Sdoi
10.1016/j.febslet.2010.03.034subject
Has Abstractpub_date
2010-06-03 00:00:00pages
2271-8issue
11eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00246-2journal_volume
584pub_type
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