Abstract:
:The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca2+]i gradient towards the plasma membranes. When treated with IP3, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP3-induced Ca2+ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and 45Ca2+ uptake experiment of the cell population.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tsunoda Y,Yodozawa S,Tashiro Ydoi
10.1016/0014-5793(88)80696-3subject
Has Abstractpub_date
1988-04-11 00:00:00pages
29-35issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)80696-3journal_volume
231pub_type
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