Abstract:
:Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P4). Similarly, prior depletion of Ins(1,3,4,5)P4-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P3. The EC50 for calcium release was 0.15 microM with Ins(1,4,5)P3 and 0.68 microM with Ins(1,3,4,5)P4. In conclusion, the Ins(1,3,4,5)P4-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P3-sensitive pool.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Raha S,Giri B,Bhattacharyya B,Biswas BBdoi
10.1016/0014-5793(95)00265-bsubject
Has Abstractpub_date
1995-04-10 00:00:00pages
316-8issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00265-Bjournal_volume
362pub_type
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