Insulin potentiates the transactivation potency of the glucocorticoid receptor.


:A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid RU30406. The N'- and C'-terminal parts of the GR protein are not involved in mediating the response. Insulin had no effect on GR binding to GRE but it affected both the level and the phosphorylation state of nuclear-bound GR. Thus, insulin alters the GR transactivation potency while, concomitantly, modifies the molecule at the posttranslational level.




FEBS letters


Georgakopoulos A,Tsawdaroglou N




Has Abstract


1996-03-04 00:00:00














  • Dexamethasone induces the expression of the mRNA of lipocortin 1 and 2 and the release of lipocortin 1 and 5 in differentiated, but not undifferentiated U-937 cells.

    abstract::The effect of dexamethasone on mRNA and protein synthesis of lipocortins (LCT) 1, 2 and 5 has been investigated in U-937 cells. A constitutive expression of both mRNAs and proteins was detected in undifferentiated U-937 cells. This constitutive level was increased time- and dose-dependently by incubation with phorbol ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Solito E,Raugei G,Melli M,Parente L

    更新日期:1991-10-21 00:00:00

  • Rolling in the clover: trefoil factor family (TFF)-domain peptides, cell migration and cancer.

    abstract::Trefoil factor family (TFF)-domain peptides 1-3 are mucin-associated molecules, largely found in epithelia of gastrointestinal tissues. Structurally similar, resistant to enzymatic degradation, they are up-regulated around areas of epithelial damage such as ulcers. Transgenic expression or exogenous peptide ameliorate...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Wright NA,Hoffmann W,Otto WR,Rio MC,Thim L

    更新日期:1997-05-19 00:00:00

  • Lipopolysaccharide-binding protein mediates CD14-independent intercalation of lipopolysaccharide into phospholipid membranes.

    abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schromm AB,Brandenburg K,Rietschel ET,Flad HD,Carroll SF,Seydel U

    更新日期:1996-12-16 00:00:00

  • Sequence-specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1).

    abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hwang MG,Chung IK,Kang BG,Cho MH

    更新日期:2001-08-10 00:00:00

  • Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against G alpha S/olf- and G alpha i-protein subunits.

    abstract::The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy V

    更新日期:1998-04-24 00:00:00

  • Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI.

    abstract::Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tisljar U,Wolf DH

    更新日期:1993-05-10 00:00:00

  • Induction of superoxide in glioma cell line U87 stimulated with lipopolysaccharide and interferon-gamma: ESR using a new flow-type quartz cell.

    abstract::The production of superoxide and nitric oxide induced in U87 glioma treated with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) was examined by electron spin resonance (ESR) spectroscopy using a newly designed flow-type quartz cuvette without detaching cells from the culture plate. ESR spectra of 2,2,6, 6-t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakagawa H,Moritake T,Tsuboi K,Ikota N,Ozawa T

    更新日期:2000-04-14 00:00:00

  • The cytochrome bc1 complex as an antipathogenic target.

    abstract::The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Fisher N,Meunier B,Biagini GA

    更新日期:2020-09-01 00:00:00

  • Minireview: Syndecans and their crucial roles during tissue regeneration.

    abstract::Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chung H,Multhaupt HA,Oh ES,Couchman JR

    更新日期:2016-08-01 00:00:00

  • alpha-Tomatine, the major saponin in tomato, induces programmed cell death mediated by reactive oxygen species in the fungal pathogen Fusarium oxysporum.

    abstract::The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ito S,Ihara T,Tamura H,Tanaka S,Ikeda T,Kajihara H,Dissanayake C,Abdel-Motaal FF,El-Sayed MA

    更新日期:2007-07-10 00:00:00

  • Apelin peptides block the entry of human immunodeficiency virus (HIV).

    abstract::The orphan G protein-coupled receptor APJ has been shown to be a coreceptor for human and simian immunodeficiency virus (HIV and SIV) strains. We have determined that some HIV and SIV strains use APJ as a coreceptor to infect the brain-derived NP-2/CD4 cells. Because apelin is an endogenous ligand for the APJ receptor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zou MX,Liu HY,Haraguchi Y,Soda Y,Tatemoto K,Hoshino H

    更新日期:2000-05-04 00:00:00

  • m-Calpain subunits remain associated in the presence of calcium.

    abstract::The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dutt P,Arthur JS,Croall DE,Elce JS

    更新日期:1998-10-09 00:00:00

  • Gastrin-induced DNA synthesis requires p38-MAPK activation via PKC/Ca(2+) and Src-dependent mechanisms.

    abstract::We present evidence that gastrin, binding to a G protein-coupled receptor, activates the p38-mitogen-activated protein kinase (MAPK) pathway. Blockage of protein kinase C (PKC) by GF109203X, depletion of intracellular calcium by thapsigargin or inhibition of Src family kinases by PP2 prevented p38-MAPK activation and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dehez S,Daulhac L,Kowalski-Chauvel A,Fourmy D,Pradayrol L,Seva C

    更新日期:2001-05-04 00:00:00

  • Growth factor receptors in helminth parasites: signalling and host-parasite relationships.

    abstract::Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Dissous C,Khayath N,Vicogne J,Capron M

    更新日期:2006-05-22 00:00:00

  • Interaction between beta-amyloid and lens alphaB-crystallin.

    abstract::In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liang JJ

    更新日期:2000-11-03 00:00:00

  • Green fluorescent protein-based halide indicators with improved chloride and iodide affinities.

    abstract::The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Galietta LJ,Haggie PM,Verkman AS

    更新日期:2001-06-22 00:00:00

  • The emergence of major cellular processes in evolution.

    abstract::The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Ouzounis C,Kyrpides N

    更新日期:1996-07-22 00:00:00

  • Human neprilysin-2 (NEP2) and NEP display distinct subcellular localisations and substrate preferences.

    abstract::Neprilysin-2 (NEP2) is a novel metallopeptidase homologous to neprilysin (NEP), an enzyme involved in regulation of neuropeptide signalling. NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2(Delta). In this study, we cloned and expressed both human isoforms. Human NEP2 exists as a membrane-bound and sol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Whyteside AR,Turner AJ

    更新日期:2008-07-09 00:00:00

  • Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boyne JR,Whitehouse A

    更新日期:2009-11-19 00:00:00

  • A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans.

    abstract::The gatC, gatA and gatB genes encoding the three subunits of glutamyl-tRNA(Gln) amidotransferase from Acidithiobacillus ferrooxidans, an acidophilic bacterium used in bioleaching of minerals, have been cloned and expressed in Escherichia coli. As in Bacillus subtilis the three gat genes are organized in an operon-like...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Salazar JC,Zúñiga R,Raczniak G,Becker H,Söll D,Orellana O

    更新日期:2001-07-06 00:00:00

  • The active sites of cellulases are involved in chiral recognition: a comparison of cellobiohydrolase 1 and endoglucanase 1.

    abstract::The cellulases cellobiohydrolase 1 (CBH 1) and endoglucanase 1 (EG 1) from the fungus Trichoderma reesei are closely related with 40% sequence identity and very similar in structure. In CBH 1 the active site is enclosed by long loops and some antiparallel beta-strands forming a 40 A long tunnel, whereas in EG 1 part o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Henriksson H,Ståhlberg J,Isaksson R,Pettersson G

    更新日期:1996-07-29 00:00:00

  • A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells.

    abstract::A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

    更新日期:1990-07-30 00:00:00

  • The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor.

    abstract::The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wei AZ,Mayr I,Bode W

    更新日期:1988-07-18 00:00:00

  • A leucine-rich repeat protein of carrot that exhibits antifreeze activity.

    abstract::A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meyer K,Keil M,Naldrett MJ

    更新日期:1999-03-26 00:00:00

  • YDL142c encodes cardiolipin synthase (Cls1p) and is non-essential for aerobic growth of Saccharomyces cerevisiae.

    abstract::The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tuller G,Hrastnik C,Achleitner G,Schiefthaler U,Klein F,Daum G

    更新日期:1998-01-02 00:00:00

  • Expression of cartilage oligomeric matrix protein by human synovium.

    abstract::Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP). Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. Weste...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Cesare PE,Carlson CS,Stollerman ES,Chen FS,Leslie M,Perris R

    更新日期:1997-07-21 00:00:00

  • New structural insights into the decoding mechanism: translation infidelity via a G·U pair with Watson-Crick geometry.

    abstract::Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Demeshkina N,Jenner L,Westhof E,Yusupov M,Yusupova G

    更新日期:2013-06-27 00:00:00

  • Nucleoside analogues previously found to be inactive against HIV may be activated by simple chemical phosphorylation.

    abstract::Nucleoside analogues previously found to be inactive against the human immunodeficiency virus (HIV) may be activated by simple chemical derivatisation. As part of our effort to deliver masked phosphates inside living cells we have discovered that certain phosphate triester derivatives of inactive nucleoside analogues ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McGuigan C,Kinchington D,Wang MF,Nicholls SR,Nickson C,Galpin S,Jeffries DJ,O'Connor TJ

    更新日期:1993-05-17 00:00:00

  • The fas locus of the phytopathogen Rhodococcus fascians affects mitosis of tobacco BY-2 cells.

    abstract::The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

    更新日期:2001-03-09 00:00:00

  • Extracellular domain of type I receptor for transforming growth factor-beta: molecular modelling using protectin (CD59) as a template.

    abstract::We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jokiranta TS,Tissari J,Teleman O,Meri S

    更新日期:1995-11-27 00:00:00