Abstract:
:A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid RU30406. The N'- and C'-terminal parts of the GR protein are not involved in mediating the response. Insulin had no effect on GR binding to GRE but it affected both the level and the phosphorylation state of nuclear-bound GR. Thus, insulin alters the GR transactivation potency while, concomitantly, modifies the molecule at the posttranslational level.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Georgakopoulos A,Tsawdaroglou Ndoi
10.1016/0014-5793(96)00115-9subject
Has Abstractpub_date
1996-03-04 00:00:00pages
177-82issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(96)00115-9journal_volume
381pub_type
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