Abstract:
:The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Harrison SM,Dove BK,Rothwell L,Kaiser P,Tarpey I,Brooks G,Hiscox JAdoi
10.1016/j.febslet.2007.02.039subject
Has Abstractpub_date
2007-04-03 00:00:00pages
1275-86issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00205-0journal_volume
581pub_type
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