Abstract:
:Treatment of HepG2 with all-trans retinoic acid (RA) induces expression of fatty acid synthase (FAS) mRNA and protein. Transfections show that the FAS promoter positively responds to retinoid X receptor (RXR) but not to RA receptor (RAR) agonists. Since RXR alone is capable of mediating the RA response of FAS, the existence of a classical RA-responsive element in the FAS promoter may be ruled out. Binding sites for NF-Y and SREBP-1 proved to be essential for the RA response. Exposure to all-trans RA increased mRNA and protein levels of SREBP-1, a transcriptional activator for FAS. Overexpression of a dominant-negative form of SREBP-1c diminished the RA-dependent increase in promoter activity. These data demonstrate that RXR ligands can stimulate the expression of a lipogenic gene solely by inducing transcription and cleavage of membrane-bound SREBP-1c.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Roder K,Zhang L,Schweizer Mdoi
10.1016/j.febslet.2007.05.022subject
Has Abstractpub_date
2007-06-12 00:00:00pages
2715-20issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00550-9journal_volume
581pub_type
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