Abstract:
:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phosphorylated by PKA and that cooperative regulation of the water channel activity of MpPIP2;1 was regulated by phosphorylation of Ser-131 of MpPIP1;1. Immunoprecipitation analysis revealed that MpPIP1;1 binds directly to MpPIP2;1 in a phosphorylation-independent manner, suggesting that phosphorylation of Ser-131 of MpPIP1;1 is involved in regulation of the structure of the channel complex with MpMIP2;1 and thereby affects water channel activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya Tdoi
10.1016/j.febslet.2005.06.082keywords:
subject
Has Abstractpub_date
2005-08-15 00:00:00pages
4417-22issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00847-1journal_volume
579pub_type
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