Primary structure of porcine muscarinic acetylcholine receptor III and antagonist binding studies.

abstract：:Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

journal_title：FEBS letters

authors： Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

更新日期：2017-09-01 00:00:00

abstract：:In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...

journal_title：FEBS letters

authors： Glitscher W,Rüppel H

更新日期：1991-05-06 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was obs...

journal_title：FEBS letters

authors： Kaether C,Gerdes HH

更新日期：1995-08-07 00:00:00

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00

abstract：:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed dow...

journal_title：FEBS letters

authors： Contzen J,Ristau O,Jung C

更新日期：1996-03-25 00:00:00

abstract：:Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM)-dependent methylation of putrescine in tropane alkaloid biosynthesis. PMT presumably evolved from the ubiquitous spermidine synthase (SPDS). SPDS protein structure suggested that only few amino acid exchanges in the active site were necessary to...

journal_title：FEBS letters

authors： Biastoff S,Reinhardt N,Reva V,Brandt W,Dräger B

更新日期：2009-10-20 00:00:00

abstract：:Proteins may undergo adaptive structural transitions to accommodate to their cellular milieu and respond to external signals. Modulation of conformational ensembles can rewire the intra- or intermolecular interaction networks and shift between different functional states. Adaptive conformational transitions are associ...

journal_title：FEBS letters

authors： Miskei M,Gregus A,Sharma R,Duro N,Zsolyomi F,Fuxreiter M

更新日期：2017-09-01 00:00:00

abstract：:Intracellular accumulation of anthracycline derivatives was measured in a human embryonic kidney cell line (HEK) and a resistant subline (HEK/multidrug resistance protein (MRP1)) overexpressing MRP1 at the plasma membrane surface. Two compounds (daunorubicin and doxorubicin) were rejected outside the multidrug-resista...

journal_title：FEBS letters

authors： Manciu L,Chang X,Riordan JR,Buyse F,Ruysschaert JM

更新日期：2001-03-23 00:00:00

abstract：:The transmission of receptor-generated cytoplasmic Ca2+ signals between communicating pancreatic acinar cells has been investigated by comparing patch-clamp recordings of Ca(2+)-dependent Cl- current in internally perfused single cells and small multi-cell clusters. Acetylcholine (50 nM) generates shortlasting repetit...

journal_title：FEBS letters

authors： Petersen CC,Petersen OH

更新日期：1991-06-17 00:00:00

abstract：:The chromatin structure in one-cell-stage mouse embryos is extremely loose and becomes tighter at the two-cell stage. As linker histones are involved in higher-order chromatin structure, we examined the involvement of the linker histone variant H1foo in the change of chromatin looseness between the one- and two-cell s...

journal_title：FEBS letters

authors： Funaya S,Ooga M,Suzuki MG,Aoki F

更新日期：2018-07-01 00:00:00

abstract：:Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36-39 and 90-93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epit...

journal_title：FEBS letters

authors： Dore JM,Morard F,Vita N,Wijdenes J

更新日期：1998-04-10 00:00:00

abstract：:The adiponectin paralog CORS-26 (collagenous repeat-containing sequence of 26kDa protein) is a member of the C1q/TNF-alpha molecular superfamily. CORS-26 is a secreted protein and baculovirus-produced CORS-26 released in the supernatant of insect cells forms stable trimers. Adiponectin exerts anti-inflammatory effects...

journal_title：FEBS letters

authors： Weigert J,Neumeier M,Schäffler A,Fleck M,Schölmerich J,Schütz C,Buechler C

更新日期：2005-10-24 00:00:00

abstract：:A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...

journal_title：FEBS letters

authors： Park CB,Lee JH,Park IY,Kim MS,Kim SC

更新日期：1997-07-14 00:00:00

abstract：:In this study, we demonstrated that tissue inhibitor of metalloproteinases (TIMP) produced by human bone marrow stromal cell line KM-102 had erythroid-potentiating activity (EPA) which stimulates the proliferation of erythroid progenitor cells. We, then, propose a scheme for the bifunctional role of TIMP/EPA in hemato...

journal_title：FEBS letters

authors： Hayakawa T,Yamashita K,Kishi J,Harigaya K

更新日期：1990-07-30 00:00:00

abstract：:Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant D...

journal_title：FEBS letters

authors： Ho T,Wang H,Pallett D,Dalmay T

更新日期：2007-07-10 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:The topic synthetic biology appears still as an 'empty basket to be filled'. However, there is already plenty of claims and visions, as well as convincing research strategies about the theme of synthetic biology. First of all, synthetic biology seems to be about the engineering of biology - about bottom-up and top-dow...

journal_title：FEBS letters

authors： Malinova V,Nallani M,Meier WP,Sinner EK

更新日期：2012-07-16 00:00:00

abstract：:The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...

journal_title：FEBS letters

authors： Wielburski A,Nelson BD

更新日期：1984-11-19 00:00:00

abstract：:The myristoylated N-terminal latching to the C-terminal lobe of c-Abl was recently demonstrated to be an important regulatory element for the kinase, playing a role similar to that of the tyrosine-phosphorylated C-terminal tail of c-Src. A potential mechanism for activating c-Abl is the dissociation of the myristoylat...

journal_title：FEBS letters

authors： Zhou HX

更新日期：2003-09-25 00:00:00

abstract：:The atomic structures of K+ channels have added a new dimension to our understanding of K+ channel function. I will briefly review how structures have influenced our views on ion conduction, gating of the pore, and voltage sensing. ...

journal_title：FEBS letters

authors： MacKinnon R

更新日期：2003-11-27 00:00:00

abstract：:The vertebrate transcription factor CTCF is not only involved in transcriptional activation, insulation and genomic imprinting, but also in transcriptional repression. Sequence motifs mediating these activities have not been identified so far. We have mapped a short repression motif to residues 150-170 within the N-te...

journal_title：FEBS letters

authors： Drueppel L,Pfleiderer K,Schmidt A,Hillen W,Berens C

更新日期：2004-08-13 00:00:00

abstract：:The roles of mild uncoupling caused by free fatty acids (mediated by plant uncoupling mitochondrial protein (PUMP) and ATP/ADP carrier (AAC)) and non-coupled respiration (alternative oxidase (AO)) on H(2)O(2) formation by plant mitochondria were examined. Both laurate and oleate prevent H(2)O(2) formation dependent on...

journal_title：FEBS letters

authors： Casolo V,Braidot E,Chiandussi E,Macrì F,Vianello A

更新日期：2000-05-26 00:00:00

abstract：:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...

journal_title：FEBS letters

authors： Van der Auwera G,Chapelle S,De Wachter R

更新日期：1994-01-31 00:00:00

abstract：:The rat chromosomal gene for uricase was cloned. The gene spans more than 20 kilobases and the coding region is divided into 8 exons by 7 introns. The organization of the rat uricase gene is so greatly different from that of the soybean gene that the difference may not have been caused only by the removal of some ance...

journal_title：FEBS letters

authors： Motojima K,Goto S

更新日期：1990-05-07 00:00:00

abstract：:The results of the present investigation relate the effects of the nutritional state and administration of clofibric acid (CLA), a hypolipidaemic drug and peroxisomal proliferator, on phosphatidylethanolamine (PE) synthesis in rat liver and fatty acid metabolism. Fasting and CLA treatment of animals causes an increase...

journal_title：FEBS letters

authors： Lenart J,Komańska I,Pikuła S,Jasińska R

更新日期：1998-08-28 00:00:00

abstract：:Co-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escherichia coli yielded fully assembled hetero-oligomeric complexes (alpha+beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the...

journal_title：FEBS letters

authors： Waldmann T,Nitsch M,Klumpp M,Baumeister W

更新日期：1995-11-27 00:00:00

abstract：:Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positivel...

journal_title：FEBS letters

authors： Sun L,Zhang T,Yu X,Xin W,Lan X,Zhang D,Huang C,Du G

更新日期：2011-09-02 00:00:00