Phosphorylation of myosin in non-muscle and smooth muscle cells. Possible rules and evolutionary trends.

abstract：:Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...

journal_title：FEBS letters

authors： Terziyska N,Lutz T,Kozany C,Mokranjac D,Mesecke N,Neupert W,Herrmann JM,Hell K

更新日期：2005-01-03 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:We have investigated the activity of STAT family members throughout a mammary developmental cycle. Transcripts for Stat 5 were upregulated during pregnancy whilst STAT1 and STAT3 mRNAs were expressed at constant levels. DNA binding complexes containing both STAT5a and 5b showed differing affinities for two naturally o...

journal_title：FEBS letters

authors： Philp JA,Burdon TG,Watson CJ

更新日期：1996-10-28 00:00:00

abstract：:The inhibitor of gamma-glutamyl transpeptidase (gamma-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of gamma-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specif...

journal_title：FEBS letters

authors： Antczak C,Karp DR,London RE,Bauvois B

更新日期：2001-11-16 00:00:00

abstract：:omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

journal_title：FEBS letters

authors： Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

更新日期：2000-03-10 00:00:00

abstract：:Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...

journal_title：FEBS letters

authors： Song M,Zhu N,Olcese R,Barila B,Toro L,Stefani E

更新日期：1999-11-05 00:00:00

abstract：:Human natural killer (NK) cells possess an arsenal of receptors programmed to regulate the NK cell functions, once encountering a target cell. In general, the activating receptors mediate cytotoxicity when engaged by their tumor specific, stress induced, virally encoded, or rarely, self ligands. Whereas, the inhibitor...

journal_title：FEBS letters

authors： Stanietsky N,Mandelboim O

更新日期：2010-12-15 00:00:00

abstract：:A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This ...

journal_title：FEBS letters

authors： Chong P,Hui I,Loo T,Gillam S

更新日期：1985-11-11 00:00:00

abstract：:The primary structure of a new sarafotoxin, SRTX-d, from the venom of Atractaspis engaddensis is described. SRTX-d differs from SRTX-b in two substitutions: Ile19 instead of Val and Thr2 instead of Ser. The toxicity of SRTX-d and its vasoconstriction potency are very low in comparison to SRTX-a and SRTX-b, whereas its...

journal_title：FEBS letters

authors： Bdolah A,Wollberg Z,Fleminger G,Kochva E

更新日期：1989-10-09 00:00:00

abstract：:Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

journal_title：FEBS letters

authors： Curty C,Engel N,Gossauer A

更新日期：1995-05-01 00:00:00

abstract：:S100 beta is a neurite extension factor and has been implicated in Alzheimer's disease and Down's syndrome. It belongs to a group of low molecular weight calcium-binding proteins containing the helix-loop-helix calcium binding motif. The structure of only one S100 protein, calbindin D9k, which has the lowest sequence ...

journal_title：FEBS letters

authors： Kilby PM,Van Eldik LJ,Roberts GC

更新日期：1995-04-17 00:00:00

abstract：:The human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obe...

journal_title：FEBS letters

authors： Walsh CJ,Guinane CM,O'Toole PW,Cotter PD

更新日期：2014-11-17 00:00:00

abstract：:Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...

journal_title：FEBS letters

authors： Okumura Y,Yano M,Murakami M,Mori S,Towatari T,Kido H

更新日期：1999-01-08 00:00:00

abstract：:In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...

journal_title：FEBS letters

authors： Pedraza CE,Baltrons MA,García A

更新日期：2001-11-02 00:00:00

abstract：:SV40 large T antigen is phosphorylated at up to ten different amino acids clustered in an N-terminal and a C-terminal part of the polypeptide chain. The N-terminal phosphorylated residues include Ser 123 and Thr 124. We have analyzed the oligomerization, the complex formation with the cellular oncoprotein p53 and the ...

journal_title：FEBS letters

authors： Montenarh M,Müller D

更新日期：1987-09-14 00:00:00

abstract：:The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...

journal_title：FEBS letters

authors： Hakoshima T

更新日期：2018-02-01 00:00:00

abstract：:Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...

journal_title：FEBS letters

authors： Mikulecky P,Andreeva E,Amara P,Weissenhorn W,Nicolet Y,Macheboeuf P

更新日期：2018-01-01 00:00:00

abstract：:During mRNA decoding at the ribosome, deviations from stringent codon identity, or "mistranslation," are generally deleterious and infrequent. Observations of organisms that decode some codons ambiguously, and the discovery of a compensatory increase in mistranslation frequency to combat environmental stress have chan...

journal_title：FEBS letters

authors： Moghal A,Mohler K,Ibba M

更新日期：2014-11-28 00:00:00

abstract：:6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) is a key enzyme of the glycolytic pathway, and it plays an essential role in angiogenesis. 3-(3-Pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) is frequently used as a glycolysis inhibitor and is thought to inhibit PFKFB3. However, this latter eff...

journal_title：FEBS letters

authors： Emini Veseli B,Perrotta P,Van Wielendaele P,Lambeir AM,Abdali A,Bellosta S,Monaco G,Bultynck G,Martinet W,De Meyer GRY

更新日期：2020-09-01 00:00:00

abstract：:We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...

journal_title：FEBS letters

authors： Scamrov AV,Beabealashvilli RS

更新日期：1983-11-28 00:00:00

abstract：:Ghrelin inhibits insulin secretion partly via induction of IA-2beta. However, the orexigenic effect of ghrelin is mediated by the AMP-activated protein kinase (AMPK)-uncoupling protein 2 (UCP2) pathway. Here, we demonstrate that ghrelin's inhibitory effect on insulin secretion also occurs through the AMPK-UCP2 pathway...

journal_title：FEBS letters

authors： Wang Y,Nishi M,Doi A,Shono T,Furukawa Y,Shimada T,Furuta H,Sasaki H,Nanjo K

更新日期：2010-04-16 00:00:00

abstract：:Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

journal_title：FEBS letters

authors： Sato T,Ishimoto T,Akiba S,Fujii T

更新日期：1993-05-24 00:00:00

abstract：:It was recently shown that there is a predominance of phase 1 introns near the cleavage site of signal peptides encoded by human genes. It was suggested that this biased distribution was due to intron insertion at AGmid R:G proto-splice sites. However, we found that there is no disproportional excess of AGmid R:G that...

journal_title：FEBS letters

authors： Vibranovski MD,Sakabe NJ,de Souza SJ

更新日期：2006-03-06 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...

journal_title：FEBS letters

authors： Ballantine JE,Woodland HR

更新日期：1985-01-28 00:00:00

abstract：:During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...

journal_title：FEBS letters

authors： de Meis L,Grieco MA,Galina A

更新日期：1992-08-17 00:00:00

abstract：:The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing ba...

journal_title：FEBS letters

authors： Arima H,Ibrahim HR,Kinoshita T,Kato A

更新日期：1997-09-22 00:00:00

abstract：:Retroviruses have been etiologically implicated with leukemia in humans and animals. Understanding the virus life cycle, the proteins and enzymes involved in its replication, is essential for developing potent anti-viral drugs. Phosphorylation of retroviral proteins may alter their shape in such a way as to increase o...

journal_title：FEBS letters

authors： Seri I,Priel E

更新日期：1996-09-02 00:00:00

abstract：:Chronic pain and epilepsy together affect hundreds of millions of people worldwide. While traditional pharmacotherapy provides essential relief to the majority of patients, a large proportion remains resistant, and surgical intervention is only possible for a select few. As both disorders are characterised by neuronal...

journal_title：FEBS letters

authors： Snowball A,Schorge S

更新日期：2015-06-22 00:00:00