Abstract:
:Ghrelin inhibits insulin secretion partly via induction of IA-2beta. However, the orexigenic effect of ghrelin is mediated by the AMP-activated protein kinase (AMPK)-uncoupling protein 2 (UCP2) pathway. Here, we demonstrate that ghrelin's inhibitory effect on insulin secretion also occurs through the AMPK-UCP2 pathway. Ghrelin increased AMPK phosphorylation and UCP2 mRNA expression in MIN6 insulinoma cells. Overexpression or downregulation of UCP2 attenuated or enhanced insulin secretion, respectively. Furthermore, AMPK activator had a similar effect to ghrelin on UCP2 and insulin secretion in MIN6 cells. In conclusion, ghrelin's inhibitory effect on insulin secretion is partly mediated by the AMPK-UCP2 pathway, which is independent of the IA-2beta pathway.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang Y,Nishi M,Doi A,Shono T,Furukawa Y,Shimada T,Furuta H,Sasaki H,Nanjo Kdoi
10.1016/j.febslet.2010.02.069subject
Has Abstractpub_date
2010-04-16 00:00:00pages
1503-8issue
8eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00173-0journal_volume
584pub_type
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