Abstract:
:Toll-like receptors (TLRs) are crucial components of the innate immune system, coupling pathogen recognition to a cellular response. We used the MAPPIT mammalian two-hybrid technique to investigate protein-protein interactions in the early steps in TLR signalling. A partial TLR-adaptor interaction map was constructed confirming several known but also documenting novel interactions. We show that the TLR adaptor Mal is critical for linking Myeloid Differentiation primary response protein 88 (MyD88) to TLR2 and TLR4. Analysis of the contributions of the different sub-domains of MyD88-adaptor-like protein (Mal) and MyD88 in adaptor homo- and hetero-dimerisation provides an initial mechanistic insight in this bridging function of Mal.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ulrichts P,Peelman F,Beyaert R,Tavernier Jdoi
10.1016/j.febslet.2007.01.026subject
Has Abstractpub_date
2007-02-20 00:00:00pages
629-36issue
4eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00050-6journal_volume
581pub_type
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