Abstract:
:Cultured rat hepatocytes exhibit acid phospholipase A activity. On the basis of product formation from stereospecifically radiolabeled phosphatidylethanolamine substrates, phospholipases A1 and A2 have been identified with optimal activities at pH 4.5. According to subcellular fractionation studies, the acid phospholipases in hepatocytes appear to be located in the lysosomal compartment. Application of specific inhibitors of the biosynthesis, glycosylation, and translocation of lysosomal enzymes in hepatocyte cultures suggests a half-life of approx. 1 day for the acid lysosomal phospholipase A1. About the same value for the half-life was obtained for the lysosomal marker enzymes, acid phosphatase and beta-N-acetyl-D-hexosaminidase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kunze H,Löffler BM,Schmidt Mdoi
10.1016/0014-5793(88)80062-0subject
Has Abstractpub_date
1988-08-29 00:00:00pages
388-90issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(88)80062-0journal_volume
236pub_type
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