A mutational analysis of active site residues in trans-3-chloroacrylic acid dehalogenase.

abstract：:Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (contr...

journal_title：FEBS letters

authors： Metz S,Holmes D,Robertson RP,Leitner W,Draznin B

更新日期：1991-12-16 00:00:00

abstract：:To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and ...

journal_title：FEBS letters

authors： Shiota G,Kunisada T,Oyama K,Udagawa A,Nomi T,Tanaka K,Tsutsumi A,Isono M,Nakamura T,Hamada H,Sakatani T,Sell S,Sato K,Ito H,Kawasaki H

更新日期：2000-03-31 00:00:00

abstract：:The formation of coenzyme A thiol esters of cinnamic, p-coumaric, p-methoxy cinnamic, and ferulic acids was catalyzed by enzyme preparations from cell suspension cultures of leaf petioles from parsley (Petroselinum hortense Hoffm.). Of these acids, p-coumaric acid served as the most efficient substrate. Enzyme activit...

journal_title：FEBS letters

authors： Hahlbrock K,Grisebach H

更新日期：1970-11-09 00:00:00

abstract：:Transient receptor potential (TRP) proteins are sensory-related cation channels. TRPV subfamily responds to vanilloids, generating a Ca(2+) current. TRPV1, a thermal-sensitive non-selective ion channel, possesses six transmembrane helices and the intracellular N- and C-terminal domains. The latter contains the PIP(2) ...

journal_title：FEBS letters

authors： Aguado-Llera D,Bacarizo J,Gregorio-Teruel L,Taberner FJ,Cámara-Artigas A,Neira JL

更新日期：2012-04-24 00:00:00

abstract：:AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...

journal_title：FEBS letters

authors： Zhang W,Xu Y,Chen Z,Xu Z,Xu H

更新日期：2011-10-03 00:00:00

abstract：:Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio...

journal_title：FEBS letters

authors： Petibois C,Drogat B,Bikfalvi A,Déléris G,Moenner M

更新日期：2007-11-27 00:00:00

abstract：:Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...

journal_title：FEBS letters

authors： Park JY,Kawada T,Han IS,Kim BS,Goto T,Takahashi N,Fushiki T,Kurata T,Yu R

更新日期：2004-08-13 00:00:00

abstract：:As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...

journal_title：FEBS letters

authors： Caner S,Zhang X,Jiang J,Chen HM,Nguyen NT,Overkleeft H,Brayer GD,Withers SG

更新日期：2016-04-01 00:00:00

abstract：:Recently mutations in the LGI1 (leucine-rich, glioma-inactivated 1) gene have been found in human temporal lobe epilepsy. We have now identified three formerly unknown LGI-like genes. Hydropathy plots and pattern analysis showed that LGI genes encode proteins with large extra- and intracellular domains connected by a ...

journal_title：FEBS letters

authors： Gu W,Wevers A,Schröder H,Grzeschik KH,Derst C,Brodtkorb E,de Vos R,Steinlein OK

更新日期：2002-05-22 00:00:00

abstract：:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...

journal_title：FEBS letters

authors： Nakano K,Arai R,Mabuchi I

更新日期：2005-09-26 00:00:00

abstract：:The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...

journal_title：FEBS letters

authors： Sakamoto C,Williams JA,Wong KY,Goldfine ID

更新日期：1983-01-10 00:00:00

abstract：:Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

journal_title：FEBS letters

authors： Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

更新日期：2017-07-01 00:00:00

abstract：:The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...

journal_title：FEBS letters

authors： Berman HM,Coimbatore Narayanan B,Di Costanzo L,Dutta S,Ghosh S,Hudson BP,Lawson CL,Peisach E,Prlić A,Rose PW,Shao C,Yang H,Young J,Zardecki C

更新日期：2013-04-17 00:00:00

abstract：:The atomic structures of K+ channels have added a new dimension to our understanding of K+ channel function. I will briefly review how structures have influenced our views on ion conduction, gating of the pore, and voltage sensing. ...

journal_title：FEBS letters

authors： MacKinnon R

更新日期：2003-11-27 00:00:00

abstract：:To unravel the mechanism of interleukin-13 (IL-13)-specific functions, we sought to identify IL-13 receptor (IL-13R) binding molecules. A novel human IL-13Ralpha1 binding protein (IL13RBP1) has been identified using yeast tri-hybrid system, which was found to encode the same protein as MIP-T3 (microtubule interacting ...

journal_title：FEBS letters

authors： Niu Y,Murata T,Watanabe K,Kawakami K,Yoshimura A,Inoue J,Puri RK,Kobayashi N

更新日期：2003-08-28 00:00:00

abstract：:Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions...

journal_title：FEBS letters

authors： Allen S,Davies J,Dawkes AC,Davies MC,Edwards JC,Parker MC,Roberts CJ,Sefton J,Tendler SJ,Williams PM

更新日期：1996-07-22 00:00:00

abstract：:We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...

journal_title：FEBS letters

authors： Jokiranta TS,Tissari J,Teleman O,Meri S

更新日期：1995-11-27 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding...

journal_title：FEBS letters

authors： Mallik S,Ray T,Kundu S

更新日期：2017-07-01 00:00:00

abstract：:CAP (c-Cbl associated protein)/ponsin belongs to a family of adaptor proteins implicated in cell adhesion and signaling. Here we show that CAP binds to and co-localizes with the essential endocytic factor dynamin. We demonstrate that CAP promotes the formation of dynamin-decorated tubule like structures, which are als...

journal_title：FEBS letters

authors： Tosoni D,Cestra G

更新日期：2009-01-22 00:00:00

abstract：:The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

journal_title：FEBS letters

authors： Zucker RM,Decal DL,Whittington KB

更新日期：1983-10-17 00:00:00

abstract：:Mod5 is a multifunctional protein that modifies a subset of tRNAs in the cytoplasm and is also required for an RNA-mediated form of transcriptional silencing. Previous in vivo studies have shown that the nuclear silencing function of Mod5 does not require that the causative tRNA gene encode a Mod5 substrate, although ...

journal_title：FEBS letters

authors： Read DF,Waller TJ,Tse E,Southworth DR,Engelke DR,Smaldino PJ

更新日期：2017-06-01 00:00:00

abstract：:Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in sing...

journal_title：FEBS letters

authors： Tarantino N,Debré P,Körner M

更新日期：1994-02-07 00:00:00

abstract：:LIMD1 is a tumour suppressor gene (TSG) down regulated in ∼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically, LIMD1 interacts with pRB, repressing E2F-driven transcription as we...

journal_title：FEBS letters

authors： Foxler DE,James V,Shelton SJ,Vallim TQ,Shaw PE,Sharp TV

更新日期：2011-04-06 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum ra...

journal_title：FEBS letters

authors： Chen HQ,Chen KP,Yao Q,Guo ZJ,Wang LL

更新日期：2007-12-22 00:00:00

abstract：:The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

journal_title：FEBS letters

authors： Creazzo TL,Wrenn RW

更新日期：1988-12-19 00:00:00

abstract：:Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...

journal_title：FEBS letters

authors： Zacharias U,He CJ,Nguyen G,Sraer JD,Rondeau E

更新日期：1993-11-15 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:Chronic exposure of a human myeloma cell line to dexamethasone resulted in a selection of cells resistant to the growth-inhibitory action of the glucocorticoid. Upon acute exposure of the parental myeloma cells to dexamethasone growth inhibition was associated with depression of ornithine decarboxylase (ODC, EC 4.1.1....

journal_title：FEBS letters

authors： Leinonen P,Alhonen-Hongisto L,Laine R,Jänne OA,Jänne J

更新日期：1987-05-04 00:00:00