Abstract:
:Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Adaptation of the SFV technology for mammalian suspension cultures has allowed the production of hundreds of milligrams of recombinant receptor for purification and structural studies. The same SFV stock solutions used for the infection of mammalian cells in culture have also been successfully applied for efficient transgene expression in organotypic hippocampal slices, as well as in vivo in rodent brain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lundstrom K,Schweitzer C,Rotmann D,Hermann D,Schneider EM,Ehrengruber MUdoi
10.1016/s0014-5793(01)02707-7keywords:
subject
Has Abstractpub_date
2001-08-31 00:00:00pages
99-103issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02707-7journal_volume
504pub_type
杂志文章,评审相关文献
FEBS LETTERS文献大全abstract::Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.049
更新日期:2006-10-02 00:00:00
abstract::During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81273-o
更新日期:1992-08-17 00:00:00
abstract::In addition to their g = 1.94 EPR signal, nitrogenase Fe-proteins from Azotobacter vinelandii, Azotobacter chroococcum and Klebsiella pneumoniae exhibit a weak EPR signal with g approximately equal to 5. Temperature dependence of the signal was consistent with an S = 3/2 system with negative zero-field splitting, D = ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81033-4
更新日期:1985-09-23 00:00:00
abstract::Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80352-5
更新日期:1985-03-25 00:00:00
abstract::We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA-DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch inf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00572-6
更新日期:2003-07-10 00:00:00
abstract::NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.006
更新日期:2008-01-23 00:00:00
abstract::We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80510-a
更新日期:1991-05-06 00:00:00
abstract::Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80560-x
更新日期:1988-10-24 00:00:00
abstract::The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.02.049
更新日期:2008-04-02 00:00:00
abstract::The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01016-9
更新日期:1999-08-20 00:00:00
abstract::By use of the signal sequence trap method, we isolated a cDNA encoding a novel aspartic protease-like protein from the mouse kidney, and termed it 'kidney-derived aspartic protease-like protein (KAP).' The protein, a 419-amino-acid polypeptide with a 16-amino-acid signal sequence, had 47% identity with mouse cathepsin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01473-1
更新日期:1997-01-20 00:00:00
abstract::Previous studies using retinal analogs (Wada, A., Sakai, M., Kinumi, T., Tsujimoto, K., Yamauchi, M. and Ito, M. (1994) J. Org. Chem. 59, 6992-6997; Wada, A., Sakai, M., Imamoto, Y., Shichida., Y., Yoshizawa, T. and Ito, M. (1993) Chem. Pharm. Bull. 41, 793-795) revealed that both retinochrome and visual pigments shar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01004-7
更新日期:1998-09-04 00:00:00
abstract::The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 microM concentration when CEM x 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM x 174 cells with 20 microM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81778-x
更新日期:1993-07-12 00:00:00
abstract::We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.048
更新日期:2006-05-29 00:00:00
abstract::Acute myeloid leukemia (AML) associated translocations often cause gene fusions that encode oncofusion proteins. Although many of the breakpoints involved in chromosomal translocations have been cloned, in most cases the role of the chimeric proteins in tumorigenesis is not elucidated. Here we will discuss the fusion ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.04.002
更新日期:2010-06-18 00:00:00
abstract::In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12105
更新日期:2016-03-01 00:00:00
abstract::A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80651-6
更新日期:1983-03-21 00:00:00
abstract::We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12332
更新日期:2016-09-01 00:00:00
abstract::We have previously shown that Rap1 as well as its guanine nucleotide exchange factor Epac1 increases cell-cell junction formation. Here, we show that activation of Epac1 with the exchange protein directly activated by cAMP (Epac)-specific cAMP analog 8CPT-2'O-Me-cAMP (007) resulted in a tightening of the junctions and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.080
更新日期:2005-09-12 00:00:00
abstract::We applied 1D homonuclear Hartmann-Hahn (1D-HOHAHA) and difference NOE experiments to determine the chemical structure of Forssman's antigen, a glycolipid purified from sheep red blood cells. The subspectra corresponding to the individual sugar components were extracted from overlapping proton resonances by selective ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81188-2
更新日期:1987-07-13 00:00:00
abstract::Alzheimer's disease is characterized by the deposition of amyloid beta-peptide (Abeta) plaques in the brain. Full-length amyloid-beta precursor protein (APP) is processed by alpha- and beta-secretases to yield soluble APP derivatives and membrane-bound C-terminal fragments, which are further processed by gamma-secreta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.003
更新日期:2007-08-07 00:00:00
abstract::Studies were designed to elucidate the mechanism by which unsaturated fatty acids inhibit the binding of triiodothyronine (T3) ro rat liver nuclei. The possibility of a direct interaction between oleic acid and T3 was excluded by dialysis experiments. Oleic acid inhibits nuclear T3 binding in a strictly competitive ma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80242-x
更新日期:1989-03-27 00:00:00
abstract::Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80479-9
更新日期:1987-11-30 00:00:00
abstract::Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80001-2
更新日期:1988-02-15 00:00:00
abstract::In this report, we show for the first time that ceramide-1-phosphate (C1P) stimulates the phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) pathway, which is a major mechanism whereby growth factors promote cell survival. Also, C1P induced IkappaB phosphorylation, and enhanced the DNA binding activity of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.067
更新日期:2005-07-04 00:00:00
abstract::The somatostatin upstream enhancer (SMS-UE) is a highly complex enhancer element. The distal A-element contains overlapping Pdx1 and Pbx binding sites. However, a point mutation in the A-element that abolishes both Pdxl and Pbx binding does not impair promoter activity. In contrast, a point mutation that selectively e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00144-1
更新日期:1999-02-26 00:00:00
abstract::Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed tha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.006
更新日期:2010-12-01 00:00:00
abstract::We have studied the effect of temperature on the contribution of Hsp104 and trehalose to barotolerance using mutants deficient in Hsp104 and trehalose synthesis. When compared with a corresponding wild type strain, mutants of Hsp104 did not show temperature dependent barotolerance when the incubation temperature durin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01141-1
更新日期:1997-10-13 00:00:00
abstract::Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01426-3
更新日期:1997-01-13 00:00:00
abstract::The final step in the biosynthesis of nicotinamide-adenine dinucleotide, a major coenzyme in cellular redox reactions and involved in intracellular signaling, is catalyzed by the enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT). The X-ray structure of human NMNAT in complex with nicotinamide mononucleoti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02556-5
更新日期:2002-04-10 00:00:00