Effect of temperature on the role of Hsp104 and trehalose in barotolerance of Saccharomyces cerevisiae.

abstract：:We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I ...

journal_title：FEBS letters

authors： Marra F,Pastacaldi S,Romanelli RG,Pinzani M,Ticali P,Carloni V,Laffi G,Gentilini P

更新日期：1997-09-08 00:00:00

abstract：:Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...

journal_title：FEBS letters

authors： Rastogi R,Verma JK,Singh V,Krishnamurthy G,Sood C,Kapoor A,Kumar K,Ansari I,Mukhopadhyay A

更新日期：2020-12-14 00:00:00

abstract：:Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...

journal_title：FEBS letters

authors： Razumovitch JA,Semenkova GN,Fuchs D,Cherenkevich SN

更新日期：2003-08-14 00:00:00

abstract：:Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...

journal_title：FEBS letters

authors： Roth B,Schwendimann B,Hughes GJ,Tzartos SJ,Barkas T

更新日期：1987-08-31 00:00:00

abstract：:The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB followed by the heavy chain variable V(H) domain, (Gly4Ser)3 linker peptide, and light chain variable V(L) domain. The correctly processed scF11 antibody was expres...

journal_title：FEBS letters

authors： Martsev SP,Kravchuk ZI,Chumanevich AA,Vlasov AP,Dubnovitsky AP,Bespalov IA,Arosio P,Deyev SM

更新日期：1998-12-28 00:00:00

abstract：:Curcumin, the active ingredient of the rhizome of Curcuma longa has anti-inflammatory, antioxidant and antiproliferative activities. Although its precise mode of action remains elusive, studies have shown that chemopreventive action of curcumin might be due to its ability to induce apoptosis in cancer cells. Curcumin ...

journal_title：FEBS letters

authors： Bhaumik S,Anjum R,Rangaraj N,Pardhasaradhi BV,Khar A

更新日期：1999-08-06 00:00:00

abstract：:Pin2/TRF1 was independently identified as a telomeric DNA-binding protein (TRF1) that regulates telomere length, and as a protein (Pin2) that can bind the mitotic kinase NIMA and suppress its lethal phenotype. We have previously demonstrated that Pin2/TRF1 levels are cell cycle-regulated and its overexpression induces...

journal_title：FEBS letters

authors： Nakamura M,Zhou XZ,Kishi S,Lu KP

更新日期：2002-03-13 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is responsible for the neutral cholesterol ester hydrolase activity in macrophages. Incubation of intact WEHI macrophages or mouse peritoneal macrophages leads to phosphorylation of HSL, which is increased by incubation with either dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine or ...

journal_title：FEBS letters

authors： Small CA,Rogers MP,Goodacre JA,Yeaman SJ

更新日期：1991-02-25 00:00:00

abstract：:A long-term goal of the field of interactome modeling is to understand how global and local properties of complex macromolecular networks impact on observable biological properties, and how changes in such properties can lead to human diseases. The information available at this stage of development of the field provid...

journal_title：FEBS letters

authors： Vidal M

更新日期：2005-03-21 00:00:00

abstract：:Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the major...

journal_title：FEBS letters

authors： Tanoue R,Kobayashi M,Katayama K,Nagata N,Wada H

更新日期：2014-05-02 00:00:00

abstract：:Lysosomal beta-galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64-d for cathepsins B, S, H, and L, and CA074Me for cathepsin B. They are converted intracellularly to active forms, E-64c and CA074, res...

journal_title：FEBS letters

authors： Okamura-Oho Y,Zhang S,Callahan JW,Murata M,Oshima A,Suzuki Y

更新日期：1997-12-15 00:00:00

abstract：:The surprising result that the spanning domain causes retention of proteins in the Golgi stack poses the question as to the actual mechanism. Here we present a simple model that might have general applicability. ...

journal_title：FEBS letters

authors： Nilsson T,Slusarewicz P,Hoe MH,Warren G

更新日期：1993-09-06 00:00:00

abstract：:Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

journal_title：FEBS letters

authors： Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

更新日期：2007-03-20 00:00:00

abstract：:Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...

journal_title：FEBS letters

authors： Vila J,Walker JM,Itarte E,Weber MJ,Sando JJ

更新日期：1989-09-11 00:00:00

abstract：:Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...

journal_title：FEBS letters

authors： Defay R,Astruc M,Beseme F,Descomps B,Crastes de Paulet A

更新日期：1984-08-06 00:00:00

abstract：:Heterotrimeric G proteins were purified from bovine brain by immunoaffinity chromatography on immobilized anti G protein monoclonal antibody 3C2. Release of betagamma subunits was effectuated by exposure of immobilized trimeric G proteins to MgAlF4. The resultant betagamma subunits were pure and biologically active. F...

journal_title：FEBS letters

authors： Rehm A,Ploegh HL

更新日期：1997-02-03 00:00:00

abstract：:Measurement of the binding equilibrium for the interaction of alpha-actinin with F-actin is complicated by secondary reactions involving cross-linking and/or bundling of the actin filaments. To quantitate the initial binding event, we studied the interaction of the bacterially expressed actin-binding domain (ABD) of c...

journal_title：FEBS letters

authors： Kuhlman PA,Ellis J,Critchley DR,Bagshaw CR

更新日期：1994-02-21 00:00:00

abstract：:Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...

journal_title：FEBS letters

authors： Vance JE

更新日期：2006-10-09 00:00:00

abstract：:RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irresp...

journal_title：FEBS letters

authors： Kizaka-Kondoh S,Okayama H

更新日期：1993-12-27 00:00:00

abstract：:Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...

journal_title：FEBS letters

authors： Loir M,Caraty A,Lanneau M,Menezo Y,Muh JP,Sautiere P

更新日期：1984-04-24 00:00:00

abstract：:Two polypeptides antigenically related to mammalian HMG1/2 have been detected in the yeast Saccharomyces cerevisiae. One exhibits an electrophoretic mobility in the range of mammalian HMG1/2 whereas the second polypeptide comigrates with yeast HMG S4. Evidence for the presence of HMG14/17 in yeast has not been obtaine...

journal_title：FEBS letters

authors： Estruch F,Pérez-Ortín JE,José M,Puigdomènech P,Franco L

更新日期：1986-03-03 00:00:00

abstract：:Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two specie...

journal_title：FEBS letters

authors： Moin K,Rozhin J,McKernan TB,Sanders VJ,Fong D,Honn KV,Sloane BF

更新日期：1989-02-13 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...

journal_title：FEBS letters

authors： Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger A

更新日期：2004-11-05 00:00:00

abstract：:Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

journal_title：FEBS letters

authors： Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

更新日期：1983-02-07 00:00:00

abstract：:Using a monoclonal antibody as conformational probe it has been shown that the weakly active nicked-beta 2 dimer of tryptophan synthase generated by proteolytic cleavage at Glu-296, does not undergo on association with alpha subunit a conformational change known to occur in intact beta 2 subunit. This alpha induced co...

journal_title：FEBS letters

authors： Linkens HJ,Djavadi-Ohaniance L,Goldberg ME

更新日期：1993-04-12 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:Stimulation of mast cells through their high affinity IgE receptor (FcεRI) leads to the secretion of pre- and neoformed soluble mediators through vesicular carriers. This process is highly regulated in order to adapt the secretion of these potentially dangerous factors to the physiological needs. This regulation requi...

journal_title：FEBS letters

authors： Benhamou M,Blank U

更新日期：2010-12-15 00:00:00

abstract：:Apomyoglobin was found to break down phosphatidylcholine vesicles to form a micellar complex at pH 4. Size reduction during this process was observed by measuring light scattering at 400 nm and by passing the complex through a gel-filtration column. Micellar complex formation was confirmed by electron microscopy. ...

journal_title：FEBS letters

authors： Lee JW,Kim H

更新日期：1988-12-05 00:00:00

abstract：:We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

journal_title：FEBS letters

authors： Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

更新日期：2003-02-11 00:00:00