Enhanced cellular uptake of biotinylated antisense oligonucleotide or peptide mediated by avidin, a cationic protein.

Abstract:

:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, which is a slightly acidic protein, did not facilitate cellular uptake. The avidin-mediated uptake of biotinylated derivatives was competitively inhibited by another cationic protein, protamine, with a Ki of 5 micrograms/ml; was saturable, temperature- and time-dependent; and was associated with endocytosis. The use of the avidin-biotin system provides a new approach to increasing the cellular uptake of antisense oligonucleotides or peptides.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Pardridge WM,Boado RJ

doi

10.1016/0014-5793(91)80996-g

subject

Has Abstract

pub_date

1991-08-19 00:00:00

pages

30-2

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(91)80996-G

journal_volume

288

pub_type

杂志文章
  • Nuclear protein transport is functionally conserved between yeast and higher eukaryotes.

    abstract::The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae, was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80121-a

    authors: Wagner P,Hall MN

    更新日期:1993-04-26 00:00:00

  • Regulation of osteopontin expression in rat mesangial cells.

    abstract::Hypercellularity and accumulation of extracellular matrix are common responses of renal glomeruli to inflammatory stimuli. Using the differential display approach, we compared the gene expression patterns of proliferating and differentiating rat mesangial cells in two- and three-dimensional cultures. Osteopontin, an e...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)01587-1

    authors: Pröls F,Heidgress D,Rupprecht HD,Marx M

    更新日期:1998-01-23 00:00:00

  • Inhibition of forskolin-stimulated cardiac adenylate cyclase activity by short-chain alcohols.

    abstract::The diterpene forskolin stimulated rat cardiac adenylate cyclase activity at least 20-fold and potentiated the effect of NaF. The stimulatory effect of forskolin was reduced in the presence of Gpp(NH)p. Ethanol markedly reduced the stimulation of adenylate cyclase by forskolin while potentiating NaF and Gpp(NH)p stimu...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80904-1

    authors: Robberecht P,Waelbroeck M,Chatelain P,Camus JC,Christophe J

    更新日期:1983-04-05 00:00:00

  • IgG immunoglobulins induce activation of the sphingomyelin cycle in HL-60 cells.

    abstract::In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00958-1

    authors: Glick D,Barenholz Y

    更新日期:1996-10-07 00:00:00

  • Unique molecular properties of superoxide dismutase from teleost fish skin.

    abstract::A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00084-m

    authors: Nakano T,Sato M,Takeuchi M

    更新日期:1995-02-27 00:00:00

  • An EVH1/WH1 domain as a key actor in TGFbeta signalling.

    abstract::EVH1 (enabled VASP (vasodilator-stimulated protein) homology 1)/WH1 (WASP (Wiskott-Aldrich syndrome protein) homology 1) domains, present in Ena VASP and WASP, are protein interaction modules specialised in binding proline-rich ligands. An EVH1/WH1 domain is here identified in the recently cloned SMIF protein, a key p...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)02751-5

    authors: Callebaut I

    更新日期:2002-05-22 00:00:00

  • The extracellular processing of HIV-1 envelope glycoprotein gp160 by human plasmin.

    abstract::Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01612-3

    authors: Okumura Y,Yano M,Murakami M,Mori S,Towatari T,Kido H

    更新日期:1999-01-08 00:00:00

  • Mia40, a novel factor for protein import into the intermembrane space of mitochondria is able to bind metal ions.

    abstract::Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.11.072

    authors: Terziyska N,Lutz T,Kozany C,Mokranjac D,Mesecke N,Neupert W,Herrmann JM,Hell K

    更新日期:2005-01-03 00:00:00

  • The primary structure of rat ribosomal protein L26.

    abstract::The amino acid sequence of rat ribosomal protein L26 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Rat L26 contains 145 amino acids and has a molecular mass of 17,266 Da. Hybridization of the cDNA to digests of nuclear DNA sug...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81434-6

    authors: Paz V,Olvera J,Chan YL,Wool IG

    更新日期:1989-07-17 00:00:00

  • Phosphorylation of protein kinase C by casein kinase-1.

    abstract::Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81092-0

    authors: Vila J,Walker JM,Itarte E,Weber MJ,Sando JJ

    更新日期:1989-09-11 00:00:00

  • Crystallization of cytochrome P-450scc from bovine adrenocortical mitochondria.

    abstract::Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and p...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)81350-4

    authors: Iwamoto Y,Tsubaki M,Hiwatashi A,Ichikawa Y

    更新日期:1988-06-06 00:00:00

  • The threonine residues in MAP kinase kinase 1 phosphorylated by MAP kinase in vitro are also phosphorylated in nerve growth factor-stimulated rat phaeochromocytoma (PC12) cells.

    abstract::The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)80252-1

    authors: Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen P

    更新日期:1994-03-14 00:00:00

  • Cholesterol uptake of isolated rat hepatocytes is accelerated by several kinds of phosphatidylcholine.

    abstract::Uptake of cholesterol by isolated rat hepatocytes in a serum-free medium was remarkably enhanced by dispersion with several kinds of phosphatidylcholine. Of the various phosphatidylcholines tested, dilinoylphosphatidylcholine had the strongest accelerating effect, while dipalmitoylphosphatidylcholine was the weakest. ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)81443-0

    authors: Nagamura Y,Sasaki K,Ito M,Saito K,Ogitsu N,Ishiguro I

    更新日期:1986-07-14 00:00:00

  • Studies on the synthesis and secretion of transthyretin by the human hepatoma cell line Hep G2.

    abstract::Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80049-9

    authors: Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

    更新日期:1991-08-05 00:00:00

  • The farnesoid X receptor induces very low density lipoprotein receptor gene expression.

    abstract::The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.04.026

    authors: Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

    更新日期:2004-05-21 00:00:00

  • Na+/H+ exchange and Ca2+ influx.

    abstract::Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/0014-5793(89)81480-2

    authors: Siffert W,Akkerman JW

    更新日期:1989-12-18 00:00:00

  • Charybdotoxin blocks both Ca-activated K channels and Ca-independent voltage-gated K channels in rat brain synaptosomes.

    abstract::Charybdotoxin (ChTX), a 4.3 kDa polypeptide toxin from the venom of the scorpion Leiurus quinquestriatus, blocks both a Ca-activated K channel (IC50 approximately 15 nM) and a Ca-independent voltage-gated K channel (IC50 approximately 40 nM) in rat brain synaptosomes. These results indicate that in this preparation Ch...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80771-9

    authors: Schneider MJ,Rogowski RS,Krueger BK,Blaustein MP

    更新日期:1989-07-03 00:00:00

  • Reactivity of glutaredoxins 1, 2 and 3 from Escherichia coli and protein disulfide isomerase towards glutathionyl-mixed disulfides in ribonuclease A.

    abstract::We have examined the activity of protein disulfide isomerase (PDI) and glutaredoxin (Grx) 1, 2 and 3 from Escherichia coli to catalyze the cleavage of glutathionylated ribonuclease A (RNase-SG) by 1 mM GSH to yield reduced RNase. Apparent Km values for RNase-SG were similar, 2-10 microM, for Grx 1, 3 and PDI but Grx I...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01698-6

    authors: Lundström-Ljung J,Vlamis-Gardikas A,Aslund F,Holmgren A

    更新日期:1999-01-25 00:00:00

  • Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP.

    abstract::The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80106-q

    authors: Madern D,Anselme J,Härtlein M

    更新日期:1992-03-24 00:00:00

  • AP-site cleavage activity of tyrosyl-DNA phosphodiesterase 1.

    abstract::APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.01.032

    authors: Lebedeva NA,Rechkunova NI,Lavrik OI

    更新日期:2011-02-18 00:00:00

  • Up-regulation of p21(WAF1/CIP1) by miRNAs and its implications in bladder cancer cells.

    abstract::We have previously reported that synthetic dsRNA can activate p21 expression by targeting the p21 promoter, thereby suppressing the proliferation of human bladder cancer cells. As complementarity between dsRNA and its target sequences is necessary for RNA activation, miRNAs may also trigger p21 expression through the ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2014.10.037

    authors: Wang C,Chen Z,Ge Q,Hu J,Li F,Hu J,Xu H,Ye Z,Li LC

    更新日期:2014-12-20 00:00:00

  • Relative contribution of Ca2+-dependent and Ca2+-independent mechanisms to the regulation of insulin secretion by glucose.

    abstract::Although insulin secretion is usually regarded as a Ca2+-dependent mechanism, recent studies have suggested the existence of a Ca2+-independent pathway of regulation by glucose. Here, mouse islets were used to compare the contribution of Ca2+-dependent and -independent pathways. Glucose increased insulin release in a ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)01547-0

    authors: Sato Y,Nenquin M,Henquin JC

    更新日期:1998-01-09 00:00:00

  • Selective inhibition of the mitochondrial permeability transition pore at the oxidation-reduction sensitive dithiol by monobromobimane.

    abstract::In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00256-9

    authors: Costantini P,Chernyak BV,Petronilli V,Bernardi P

    更新日期:1995-04-03 00:00:00

  • Involvement of Na+/H+ exchangers in induction of cyclooxygenase-2 by vacuolar-type (H+)-ATPase inhibitors in RAW 264 cells.

    abstract::In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.08.055

    authors: Kamachi F,Yanai M,Ban HS,Ishihara K,Hong J,Ohuchi K,Hirasawa N

    更新日期:2007-10-02 00:00:00

  • The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19.

    abstract::Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81253-0

    authors: Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

    更新日期:1989-04-10 00:00:00

  • Purification of a putative Na+/D-glucose cotransporter from pig kidney brush border membranes on a phlorizin affinity column.

    abstract::Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)81315-2

    authors: Kitlar T,Morrison AI,Kinne R,Deutscher J

    更新日期:1988-07-04 00:00:00

  • Na+,K(+)-ATPase of gastric cells. A target of Helicobacter pylori cytotoxic activity.

    abstract::The present study shows a direct impairing action of a cytotoxin-producing Helicobacter pylori strain on the Na+,K(+)-ATPase (evaluated as K(+)-dependent phosphatase activity) of human gastric epithelial cells in culture. The toxin itself is likely involved in this action which may also account for the cell edema foun...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81703-3

    authors: Ricci V,Sommi P,Cova E,Fiocca R,Romano M,Ivey KJ,Solcia E,Ventura U

    更新日期:1993-11-15 00:00:00

  • Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus.

    abstract::The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunit...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.09.062

    authors: Peng G,Bostina M,Radermacher M,Rais I,Karas M,Michel H

    更新日期:2006-10-30 00:00:00

  • Chemokine receptor inhibition by AMD3100 is strictly confined to CXCR4.

    abstract::This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03143-5

    authors: Hatse S,Princen K,Bridger G,De Clercq E,Schols D

    更新日期:2002-09-11 00:00:00

  • Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells.

    abstract::Programmed death-ligand 1 (PD-L1) is a co-inhibitory molecule expressed on tumor cells. Immune checkpoint inhibitors focusing on the PD-L1 mechanism are now being studied for the treatment of various cancer types. However, the regulatory mechanism of PD-L1 is yet to be fully clarified, and a high-throughput system for...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.14032

    authors: Uchida Y,Matsushima T,Kurimoto R,Chiba T,Inutani Y,Asahara H

    更新日期:2021-01-09 00:00:00