Abstract:
:Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis of RFP from Discosoma (DsRed) resulted in the monomeric mRFP1 and in the tetrameric RedStar RFP, which is distinguished by its enhanced fluorescence in vivo. Based on these mutations, we have generated an enhanced monomeric RFP, mRFPmars, and report its spectral properties. Together with green fluorescent labels, we used mRFPmars to visualize filamentous actin structures and microtubules in Dictyostelium cells. This enhanced RFP proved to be suitable to monitor the dynamics of cytoskeletal proteins in cell motility, mitosis, and endocytosis using dual-wavelength fluorescence microscopy.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger Adoi
10.1016/j.febslet.2004.09.084keywords:
subject
Has Abstractpub_date
2004-11-05 00:00:00pages
227-32issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304012414journal_volume
577pub_type
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