Do monoclonal antibodies Tü15 and Tü67 detect heterogeneity of human transferrin receptor molecules?

abstract：:OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipop...

journal_title：FEBS letters

authors： Arockiasamy A,Krishnaswamy S

更新日期：1999-06-25 00:00:00

abstract：:The substrate activation behaviour of pyruvate decarboxylase from germinating seeds of Pisum sativum is characterised kinetically via stopped-flow measurements and discussed with respect to other species. The involvement of SH-groups in this process is demonstrated by reference experiments with chemically modified enz...

journal_title：FEBS letters

authors： Dietrich A,König S

更新日期：1997-01-02 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:It is now widely recognized that the packaging of genomic DNA, together with core histones, linker histones, and other functional proteins into chromatin profoundly influences nuclear processes such as transcription, replication, DNA repair, and recombination. Whereas earlier structural studies portrayed nucleosomes (...

journal_title：FEBS letters

authors： Chakravarthy S,Park YJ,Chodaparambil J,Edayathumangalam RS,Luger K

更新日期：2005-02-07 00:00:00

abstract：:Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...

journal_title：FEBS letters

authors： Peterson J,Vibat C,Gennis RB

更新日期：1994-11-28 00:00:00

abstract：:Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA fo...

journal_title：FEBS letters

authors： Morikawa T,Yasuno R,Wada H

更新日期：2001-06-01 00:00:00

abstract：:Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...

journal_title：FEBS letters

authors： Hu ZZ,Tsai-Morris CH,Buczko E,Dufau ML

更新日期：1990-11-12 00:00:00

abstract：:Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...

journal_title：FEBS letters

authors： Lu C,Shi Y,Wang Z,Song Z,Zhu M,Cai Q,Chen T

更新日期：2008-08-06 00:00:00

abstract：:E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

journal_title：FEBS letters

authors： Lissin NM,Hemmingsen SM

更新日期：1993-06-07 00:00:00

abstract：:Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...

journal_title：FEBS letters

authors： Sterner R,Vogl T,Hinz HJ,Penz F,Hoff R,Föll R,Decker H

更新日期：1995-05-01 00:00:00

abstract：:A simple model is put forward to explain the long-known three-base periodicity in coding DNA. We propose the concept of same-phase triplet clustering, i.e. a condition wherein a triplet appears several times in one phase without interruption by the two other possible phases. For instance, in the sequence (i): NTT_GNN_...

journal_title：FEBS letters

authors： Sánchez J,López-Villaseñor I

更新日期：2006-11-27 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...

journal_title：FEBS letters

authors： Kumazoe M,Kim Y,Bae J,Takai M,Murata M,Suemasu Y,Sugihara K,Yamashita S,Tsukamoto S,Huang Y,Nakahara K,Yamada K,Tachibana H

更新日期：2013-09-17 00:00:00

abstract：:It is known that chemokine-like factor superfamily 8 (CKLFSF8), a member of the CKLF superfamily, has four putative transmembrane regions and a MARVEL domain. Its structure is similar to TM4SF11 (plasmolipin) and widely distributed in normal tissue. However, its function is not yet known. We show here that CKLFSF8 is ...

journal_title：FEBS letters

authors： Jin C,Ding P,Wang Y,Ma D

更新日期：2005-11-21 00:00:00

abstract：:Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important prote...

journal_title：FEBS letters

authors： Zhang Y,Stevens JM,Ferguson SJ

更新日期：2014-09-17 00:00:00

abstract：:A homogenate of purified chromaffin cells was fractionated, after removal of the nuclear fraction, by sucrose density gradient ultracentrifugation. The presence and subcellular localization of low molecular mass GTP-binding proteins was explored by incubation of blots of proteins from different subcellular fractions w...

journal_title：FEBS letters

authors： Doucet JP,Fournier S,Parulekar M,Trifaró JM

更新日期：1989-04-10 00:00:00

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...

journal_title：FEBS letters

authors： Vogel LK,Norén O,Sjöström H

更新日期：1992-08-10 00:00:00

abstract：:During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...

journal_title：FEBS letters

authors： Burnol AF,Guerre-Millo M,Lavau M,Girard J

更新日期：1986-01-06 00:00:00

abstract：:The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

journal_title：FEBS letters

authors： Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

更新日期：1985-01-01 00:00:00

abstract：:Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

journal_title：FEBS letters

authors： van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

更新日期：1999-10-22 00:00:00

abstract：:A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...

journal_title：FEBS letters

authors： Janecek S

更新日期：1995-12-11 00:00:00

abstract：:Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human ...

journal_title：FEBS letters

authors： Minet E,Arnould T,Michel G,Roland I,Mottet D,Raes M,Remacle J,Michiels C

更新日期：2000-02-18 00:00:00

abstract：:Amylin, a 37 amino acid C-terminal amidated peptide is an integral part of secretory granules of pancreatic beta-cells. Utilizing a specific radioimmunoassay system we demonstrate in the present study a cosecretion of amylin and insulin from the isolated rat pancreas. The secretion pattern of both peptides during gluc...

journal_title：FEBS letters

authors： Fehmann HC,Weber V,Göke R,Göke B,Arnold R

更新日期：1990-03-26 00:00:00

abstract：:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to...

journal_title：FEBS letters

authors： Breuer W,Epsztejn S,Cabantchik ZI

更新日期：1996-03-18 00:00:00

abstract：:A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...

journal_title：FEBS letters

authors： Fournel-Gigleux S,Jackson MR,Wooster R,Burchell B

更新日期：1989-01-30 00:00:00

abstract：:A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...

journal_title：FEBS letters

authors： Langbakk B,Flatmark T

更新日期：1984-09-03 00:00:00

abstract：:The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigme...

journal_title：FEBS letters

authors： Zhang Y,Nakamura A,Kuroiwa Y,Kato Y,Watanabe T

更新日期：2008-04-02 00:00:00

abstract：:The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...

journal_title：FEBS letters

authors： Evans LD,Roszak AW,Noble LJ,Robinson DA,Chalk PA,Matthews JL,Coggins JR,Price NC,Lapthorn AJ

更新日期：2002-10-23 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:Plant Golgi bodies possess unique morphological and functional characteristics that are key to several biological and biotechnological processes, such as transport of the cell's building blocks to energy-rich compartments, including chloroplasts, storage vacuoles and a cellulosic cell wall. During the last decade it h...

journal_title：FEBS letters

authors： Faso C,Boulaflous A,Brandizzi F

更新日期：2009-12-03 00:00:00