Stereospecificity in enzymic and non-enzymic oxidation of beta-O-4 lignin model compounds.

abstract：:The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...

journal_title：FEBS letters

authors： Ianaro A,Maffia P,Cuzzocrea S,Mazzon E,Santoro MG,Di Rosa M,Ialenti A

更新日期：2003-10-09 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:Heat shock (44 degrees C for 10 min) or ATP depletion by an uncoupler (CCCP for 20 min) is shown to result in stimulation of hsp68/70 synthesis in Ehrlich tumor cells. After 3 h of recovery, the cells become thermotolerant. Surprisingly, repeated ATP depletion caused by CCCP or rotenone (a respiratory inhibitor) treat...

journal_title：FEBS letters

authors： Gabai VL,Kabakov AE

更新日期：1993-08-02 00:00:00

abstract：:Microdissected, beta-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86Rb+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ou...

journal_title：FEBS letters

authors： Lindström P,Norlund L,Sehlin J

更新日期：1986-05-05 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...

journal_title：FEBS letters

authors： Hamada M,Kuwabara T,Warashina M,Nakayama A,Taira K

更新日期：1999-11-12 00:00:00

abstract：:The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequen...

journal_title：FEBS letters

authors： Mohr E,Richter D

更新日期：1990-01-29 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:In this study, we demonstrated that tissue inhibitor of metalloproteinases (TIMP) produced by human bone marrow stromal cell line KM-102 had erythroid-potentiating activity (EPA) which stimulates the proliferation of erythroid progenitor cells. We, then, propose a scheme for the bifunctional role of TIMP/EPA in hemato...

journal_title：FEBS letters

authors： Hayakawa T,Yamashita K,Kishi J,Harigaya K

更新日期：1990-07-30 00:00:00

abstract：:A major difference between porcine and bovine pancreatic phospholipase A2 (PA2) is the relatively low affinity of the bovine enzyme for lipid-water interfaces. We have investigated the binding of porcine, bovine, and equine PA2 to n-hexadecylphosphocholine (C16-PC) micelles using optically detected magnetic resonance ...

journal_title：FEBS letters

authors： Mao SY,Maki AH,de Haas GH

更新日期：1987-01-19 00:00:00

abstract：:Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...

journal_title：FEBS letters

authors： Mikulecky P,Andreeva E,Amara P,Weissenhorn W,Nicolet Y,Macheboeuf P

更新日期：2018-01-01 00:00:00

abstract：:H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

journal_title：FEBS letters

authors： Vygodina T,Konstantinov AA

更新日期：1987-07-27 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00

abstract：:A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazo...

journal_title：FEBS letters

authors： Villanueva VR,Lederer E

更新日期：1975-04-01 00:00:00

abstract：:We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO(-)) by detection of the reaction products. Pel was reacted with ONOO(-), then the reaction mi...

journal_title：FEBS letters

authors： Tsuda T,Kato Y,Osawa T

更新日期：2000-11-10 00:00:00

abstract：:The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunit...

journal_title：FEBS letters

authors： Peng G,Bostina M,Radermacher M,Rais I,Karas M,Michel H

更新日期：2006-10-30 00:00:00

abstract：:Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...

journal_title：FEBS letters

authors： Guinec N,Dalet-Fumeron V,Pagano M

更新日期：1992-08-24 00:00:00

abstract：:We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...

journal_title：FEBS letters

authors： Lapidot A,Vijayabaskar V,Litovchick A,Yu J,James TL

更新日期：2004-11-19 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

journal_title：FEBS letters

authors： Loomes KM,Senior HE

更新日期：1997-04-01 00:00:00

abstract：:The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

journal_title：FEBS letters

authors： Comis A,Easterbrook-Smith SB

更新日期：1986-03-03 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects ...

journal_title：FEBS letters

authors： Yoshida T,Klinkspoor JH,Kuver R,Wrenn SP,Kaler EW,Lee SP

更新日期：2000-07-28 00:00:00

abstract：:The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...

journal_title：FEBS letters

authors： Lilie H,Buchner J

更新日期：1995-03-27 00:00:00

abstract：:Engrailed-2 (En-2) belongs to an evolutionarily conserved family of DNA binding homeodomain-containing proteins that are expressed in mammalian brain during development. Here, we demonstrate that serine 267 in the homeodomain of En-2 is phosphorylated by protein kinase A (PKA) in forskolin-treated COS-7 cells. Further...

journal_title：FEBS letters

authors： Hjerrild M,Stensballe A,Jensen ON,Gammeltoft S,Rasmussen TE

更新日期：2004-06-18 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...

journal_title：FEBS letters

authors： Karino A,Tanoue S,Fukuda M,Nakamura T,Ohtsuki K

更新日期：1996-12-02 00:00:00

abstract：:Cloning and nucleotide sequence analysis of the human calcitonin mRNA from the BEN lung carcinoma cell line, a cell line known to secrete high-Mr forms of calcitonin, showed no difference in the coding region at the nucleotide level compared with calcitonin mRNA isolated from medullary thyroid carcinoma which secretes...

journal_title：FEBS letters

authors： Riley JH,Edbrooke MR,Craig RK

更新日期：1986-03-17 00:00:00

abstract：:A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da...

journal_title：FEBS letters

authors： Ledesma A,Villalba M,Rodríguez R

更新日期：2000-01-21 00:00:00

abstract：:Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O2-), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (.OH) scavengers inh...

journal_title：FEBS letters

authors： Inoue S,Kawanishi S

更新日期：1995-08-28 00:00:00

abstract：:Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The deg...

journal_title：FEBS letters

authors： Hoshino J,Nothacker HP,Schalge S,Kröger H

更新日期：1990-03-12 00:00:00