Glucose reduces both Rb+ influx and efflux in pancreatic islet cells.

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the a...

journal_title：FEBS letters

authors： Nagahama M,Nakayama K,Hori H,Murakami K

更新日期：1989-12-18 00:00:00

abstract：:The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase r...

journal_title：FEBS letters

authors： Banas B,Eberle J,Banas B,Schlöndorff D,Luckow B

更新日期：2001-12-07 00:00:00

abstract：:Two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) was used to assign the proton resonances of ferricytochrome C553 from Desulfovibrio vulgaris Hildenborough. The spin systems of 76 out of 79 amino acids were identified by J-correlation spectroscopy (COSY and HOHAHA) in H20 and D20 and correlated by nucl...

journal_title：FEBS letters

authors： Blanchard L,Blackledge MJ,Marion D,Guerlesquin F

更新日期：1996-07-01 00:00:00

abstract：:The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

journal_title：FEBS letters

authors： Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

更新日期：1999-06-11 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...

journal_title：FEBS letters

authors： Lee KW,Yim HS,Shin J,Lee C,Lee JH,Jeong JY

更新日期：2017-01-01 00:00:00

abstract：:Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

journal_title：FEBS letters

authors： Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

更新日期：2003-06-19 00:00:00

abstract：:Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...

journal_title：FEBS letters

authors： Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger A

更新日期：2004-11-05 00:00:00

abstract：:It is clear that G1-S phase control is exerted after the mouse embryo implants into the uterus 4.5 days after fertilization (E4.5); null mutants of genes that control cell cycle commitment such as max, rb (retinoblastoma), and dp1 are embryonic lethal after implantation with proliferation phenotypes. But, a number of ...

journal_title：FEBS letters

authors： Xie Y,Sun T,Wang QT,Wang Y,Wang F,Puscheck E,Rappolee DA

更新日期：2005-01-17 00:00:00

abstract：:Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in...

journal_title：FEBS letters

authors： Hikosaka A,Takaya K,Jinno M,Kawahara A

更新日期：2007-06-26 00:00:00

abstract：:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...

journal_title：FEBS letters

authors： van Kleef MA,Duine JA

更新日期：1988-09-12 00:00:00

abstract：:We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-b...

journal_title：FEBS letters

authors： Kase R,Kitagawa H,Hayashi K,Tanoue K,Inagaki F

更新日期：1989-08-28 00:00:00

abstract：:NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

journal_title：FEBS letters

authors： Murray JT,Cummings LA,Bloomberg GB,Cohen P

更新日期：2005-02-14 00:00:00

abstract：:In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...

journal_title：FEBS letters

authors： Kunishima N,Amada F,Fukuyama K,Kawamoto M,Matsunaga T,Matsubara H

更新日期：1996-01-15 00:00:00

abstract：:The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...

journal_title：FEBS letters

authors： Olsen J,Cowell GM,Kønigshøfer E,Danielsen EM,Møller J,Laustsen L,Hansen OC,Welinder KG,Engberg J,Hunziker W

更新日期：1988-10-10 00:00:00

abstract：:In prokaryotic and eukaryotic organisms, proteins are efficiently sorted to reach their final destinations in a whole range of subcellular compartments. Targeting is mediated by hydrophobic signal sequences or hydrophilic targeting sequences depending upon the compartment, these sequences being often processed. Protei...

journal_title：FEBS letters

authors： Lazdunski CJ,Benedetti H

更新日期：1990-08-01 00:00:00

abstract：:The mechanisms controlling fat depot-specific metabolism are poorly understood. During starvation of mice, downregulation of lipogenic genes, suppression of fatty acid synthesis, and increases in lipid oxidation were all more pronounced in epididymal than in subcutaneous fat. In epididymal fat, relatively strong upreg...

journal_title：FEBS letters

authors： Sponarova J,Mustard KJ,Horakova O,Flachs P,Rossmeisl M,Brauner P,Bardova K,Thomason-Hughes M,Braunerova R,Janovska P,Hardie DG,Kopecky J

更新日期：2005-11-07 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensi...

journal_title：FEBS letters

authors： Kimura T,Nishikawa M,Fujisawa J

更新日期：1996-07-15 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...

journal_title：FEBS letters

authors： Michot B,Despres L,Bonhomme F,Bachellerie JP

更新日期：1993-02-01 00:00:00

abstract：:Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, in freshly extracted cellular DNA and in plant cell extracts. We have also found that kinetin has electrochemical properties which can be used for monitoring the level of this modified base in DNA. Here, for the firs...

journal_title：FEBS letters

authors： Barciszewski J,Siboska GE,Pedersen BO,Clark BF,Rattan SI

更新日期：1997-09-08 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...

journal_title：FEBS letters

authors： Brzezinski P,Thörnström PE,Malmström BG

更新日期：1986-01-01 00:00:00

abstract：:Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...

journal_title：FEBS letters

authors： Zaba BN,Wilkinson MC,Taylor DM,Lewis TJ,Laidman DL

更新日期：1987-03-09 00:00:00

abstract：:The matrix protein M of Borna disease virus (BDV) is a constituent of the viral envelope covering the inner leaflet of the lipid bilayer. BDV-M was expressed as recombinant protein in Escherichia coli, purified to homogeneity and structurally analyzed. Recombinant M (i) forms non-covalently bound multimers with a Stok...

journal_title：FEBS letters

authors： Kraus I,Bogner E,Lilie H,Eickmann M,Garten W

更新日期：2005-05-09 00:00:00

abstract：:Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

journal_title：FEBS letters

authors： Afanas'ev IB,Ostrachovich EA,Korkina LG

更新日期：1998-03-27 00:00:00

abstract：:Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...

journal_title：FEBS letters

authors： Vilhjálmsdóttir J,Albertsson I,Blomberg MRA,Ädelroth P,Brzezinski P

更新日期：2020-03-01 00:00:00