Abstract:
:The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase reporter gene constructs identified the minimal DNA sequence mediating the negative regulatory effect of cAMP on HIV-1 transcription. A single nuclear factor kappaB element from the HIV-1 enhancer mediates the repressive effect on transcription. AP-2 is not involved in cAMP repression. Stable transfection of Jurkat T cells with the co-activators CREB binding protein (CBP) and p300 completely abolished the cAMP repressive effect, supporting the hypothesis that elevation of intracellular cAMP increases phosphorylation of CREB, which then competes with phosphorylated p65 and Ets-1 for limiting amounts of CBP/p300 thereby mediating the observed repressive effect on transcription. These findings suggest an important role of cAMP on HIV-1 transcription.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Banas B,Eberle J,Banas B,Schlöndorff D,Luckow Bdoi
10.1016/s0014-5793(01)03182-9keywords:
subject
Has Abstractpub_date
2001-12-07 00:00:00pages
207-12issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)03182-9journal_volume
509pub_type
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