Abstract:
:The amino acid sequence of EcoRV DNA methyltransferase which methylates the amino group of the 5'-adenine residue of the target sequence GATATC has been found to be closely related to that of three other adenine methyltransferases, DpnII, dam and damT4, the target sequence of which is GATC. Despite large differences on the DNA level, the four sequences show four blocks of homologies. One of these blocks has the sequence DVYXDPPY and is found with little modification in numerous other DNA methyltransferases. It is speculated that it could be the binding site of the methyl donor, S-adenosylmethionine. On the other hand, the identification of a DNA-binding region is more tenuous. As expected, no analogies with (dimeric) repressors and cro proteins which have the characteristic helix-turn-helix motif have been observed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lauster R,Kriebardis A,Guschlbauer Wdoi
10.1016/0014-5793(87)80897-9subject
Has Abstractpub_date
1987-08-10 00:00:00pages
167-76issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80897-9journal_volume
220pub_type
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