Cdc48 can distinguish between native and non-native proteins in the absence of cofactors.


:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substrates. First, Cdc48 prevents luciferase from aggregating following a heat shock. Second, it inhibits the aggregation of rhodanese upon dilution. Third, Cdc48 binds specifically to heat-denatured luciferase. These chaperone-like functions seem to be independent of ATPase activity. Furthermore, Cdc48 can act as a co-chaperone in the Hsc70-Hsp40 chaperone system. These results show that Cdc48 possesses chaperone-like activities and can functionally interact with Hsc70. Cdc48's ability to recognise denatured proteins can also be a source of unspecific binding in biochemical interaction experiments.




FEBS letters


Thoms S





Has Abstract


2002-06-05 00:00:00














  • Annexin-I inhibits phospholipase A2 by specific interaction, not by substrate depletion.

    abstract::Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim KM,Kim DK,Park YM,Kim CK,Na DS

    更新日期:1994-05-02 00:00:00

  • Cooperativity between platelet-activating factor and collagen in aggregation of bovine platelets III.

    abstract::In cooperative aggregation of bovine platelets induced by simultaneous addition of PAF and collagen at subthreshold concentrations the following observations were made. (i) Formation of phosphatidic acid and arachidonic acid metabolites, which characterize PAF and collagen alone-induced aggregation, respectively, was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kojima S,Sekiya F,Inada Y,Sato F,Tsukada T,Saito Y

    更新日期:1990-07-16 00:00:00

  • Downregulation of collagen synthesis in fibroblasts within three-dimensional collagen lattices involves transcriptional and posttranscriptional mechanisms.

    abstract::Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eckes B,Mauch C,Hüppe G,Krieg T

    更新日期:1993-03-01 00:00:00

  • Functional expression of human cone pigments using recombinant baculovirus: compatibility with histidine tagging and evidence for N-glycosylation.

    abstract::Mammalian color vision is mediated by light-sensitive pigments in retinal cone cells. Biochemical studies on native mammalian cone visual pigments are seriously hampered by their low levels and instability. We describe a novel approach for their functional expression, employing the baculovirus system in combination wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vissers PM,DeGrip WJ

    更新日期:1996-10-28 00:00:00

  • Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins.

    abstract::Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi T

    更新日期:2005-12-19 00:00:00

  • Progranulin compensates for blocked IGF-1 signaling to promote myotube hypertrophy in C2C12 myoblasts via the PI3K/Akt/mTOR pathway.

    abstract::It is well known that growth hormone (GH)-induced IGF-1 signaling plays a dominant role in postnatal muscle growth. Our previous studies have identified a growth factor, progranulin (PGRN), that is co-induced with IGF-1 upon GH administration. This result prompted us to explore the function of PGRN and its association...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hu SY,Tai CC,Li YH,Wu JL

    更新日期:2012-09-21 00:00:00

  • Requirements for a conservative protein translocation pathway in chloroplasts.

    abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vojta L,Soll J,Bölter B

    更新日期:2007-06-12 00:00:00

  • The cis-element CE-LPH1 of the rat intestinal lactase gene promoter interacts in vitro with several nuclear factors present in endodermal tissues.

    abstract::We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boukamel R,Freund JN

    更新日期:1994-10-10 00:00:00

  • Molecular cloning, characterization and evolution of the gene encoding a new group of short-chain alpha-neurotoxins in an Australian elapid, Pseudonaja textilis.

    abstract::The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain alpha-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a res...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gong N,Armugam A,Jeyaseelan K

    更新日期:2000-05-19 00:00:00

  • Investigation of the interaction of the water oxidising manganese complex of photosystem II with the aqueous solvent environment.

    abstract::Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Evans MC,Gourovskaya K,Nugent JH

    更新日期:1999-05-07 00:00:00

  • 1,2-alpha-l-Fucosynthase: a glycosynthase derived from an inverting alpha-glycosidase with an unusual reaction mechanism.

    abstract::Fucosyloligosaccharides have great therapeutic potential. Here we present a new route for synthesizing a Fucalpha1,2Gal linkage by introducing glycosynthase technology into 1,2-alpha-l-fucosidase. The enzyme adopts a unique reaction mechanism, in which asparagine-423 activated by aspartic acid-766 acts as a base while...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wada J,Honda Y,Nagae M,Kato R,Wakatsuki S,Katayama T,Taniguchi H,Kumagai H,Kitaoka M,Yamamoto K

    更新日期:2008-11-12 00:00:00

  • COP9 signalosome function in the DDR.

    abstract::The COP9 signalosome (CSN) is a platform for protein communication in eukaryotic cells. It has an intrinsic metalloprotease that removes the ubiquitin (Ub)-like protein Nedd8 from cullins. CSN-mediated deneddylation regulates culling-RING Ub ligases (CRLs) and controls ubiquitination of proteins involved in DNA damage...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hannss R,Dubiel W

    更新日期:2011-09-16 00:00:00

  • Carbohydrate-induced modulation of cell membrane. VIII. Agglutination with mammalian lectin galectin-1 increases osmofragility and membrane fluidity of trypsinized erythrocytes.

    abstract::Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

    更新日期:2006-03-06 00:00:00

  • Evidence for two H2O2-binding sites in ferric cytochrome c oxidase. Indication to the O-cycle?

    abstract::H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vygodina T,Konstantinov AA

    更新日期:1987-07-27 00:00:00

  • Voltage sensor mutations differentially target misfolded K+ channel subunits to proteasomal and non-proteasomal disposal pathways.

    abstract::In Shaker K(+) channels, formation of an electrostatic interaction between two charged residues, D316 and K374 in transmembrane segments S3 and S4, respectively, is a key step in voltage sensor biogenesis. Mutations D316K and K374E disrupt formation of the voltage sensor and lead to endoplasmic reticulum retention. We...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Myers MP,Khanna R,Lee EJ,Papazian DM

    更新日期:2004-06-18 00:00:00

  • Stimulation and inhibition of human platelet membrane high-affinity GTPase by neomycin.

    abstract::The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Herrmann E,Jakobs KH

    更新日期:1988-02-29 00:00:00

  • Uber die quantitative Bestimmung von Sterinderivaten in pflanzen und die intrazelluläre Verteilung der Steringlycoside in Blättern.

    abstract::The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eichenberger W,Grob EC

    更新日期:1970-12-01 00:00:00

  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.

    abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

    更新日期:2003-01-02 00:00:00

  • Peroxynitrite causes calcium efflux from mitochondria which is prevented by Cyclosporin A.

    abstract::Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Packer MA,Murphy MP

    更新日期:1994-05-30 00:00:00

  • Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.

    abstract::A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

    更新日期:1993-03-15 00:00:00

  • Targeted knock-out of a gene encoding sulfite reductase in the moss Physcomitrella patens affects gametophytic and sporophytic development.

    abstract::A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva S

    更新日期:2010-06-03 00:00:00

  • Gene relaxation and aging: changes in the abundance of rat ventral prostate SGP-2 (clusterin) and ornithine decarboxylase mRNAs.

    abstract::Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

    更新日期:1994-07-18 00:00:00

  • Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor.

    abstract::Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially throu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

    更新日期:2014-06-13 00:00:00

  • Up-regulation of lactosylceramide synthase in MDR1 overexpressing human liver tumour cells.

    abstract::HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hummel I,Klappe K,Kok JW

    更新日期:2005-06-20 00:00:00

  • Vasopressin stimulates long-term net chloride secretion in cortical collecting duct cells.

    abstract::The classical short-term effect (within minutes) of arginine vasopressin (AVP) consists in increasing sodium, chloride and water transport in kidney cells. More recently, long-term actions (several hours) of the hormone have been evidenced on water and sodium fluxes, due to transcriptional enhancement in the expressio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Djelidi S,Fay M,Cluzeaud F,Thomas-Soumarmon A,Bonvalet JP,Farman N,Blot-Chabaud M

    更新日期:1999-11-05 00:00:00

  • Characterization and purification of a hydrogenase from the eukaryotic green alga Scenedesmus obliquus.

    abstract::Several catalytic properties of the hydrogenase from Scenedesmus obliquus have been examined to optimize the purification conditions. The Km-value for H2-evolution in the presence of the most effective electron mediator methylviologen is 0.66 mM. The pH-optimum is 6.3, the temperature-optimum is 50 degrees C and the e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schnackenberg J,Schulz R,Senger H

    更新日期:1993-07-19 00:00:00

  • Cell cycle-dependent association of Gag-Mil and hsp90.

    abstract::Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lovrić J,Bischof O,Moelling K

    更新日期:1994-04-18 00:00:00

  • Apicomplexan parasites contain a single lipoic acid synthase located in the plastid.

    abstract::Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomsen-Zieger N,Schachtner J,Seeber F

    更新日期:2003-07-17 00:00:00

  • Light changes the membrane potential and ion balances of retinal rod disks.

    abstract::Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hughes SM,Brand MD

    更新日期:1985-03-25 00:00:00

  • Dietary flavonoid and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolase.

    abstract::Lactase phlorizin hydrolase (LPH; EC is a membrane-bound, family 1 beta-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k(cat)/K(m)) for the hy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Day AJ,Cañada FJ,Díaz JC,Kroon PA,Mclauchlan R,Faulds CB,Plumb GW,Morgan MR,Williamson G

    更新日期:2000-02-25 00:00:00