Characterization of topoisomerase II-DNA interaction and identification of a DNA-binding domain by ultraviolet laser crosslinking.


:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1.2 +/- 0.5 x 10(8) M(-1) was determined from kinetic analysis. Topoisomerase II showed highest affinity for supercoiled DNA. Limited proteolysis of crosslinked topoisomerase II-DNA complexes showed a site of crosslinking to be within a 29-kDa fragment with Leu-681 at its amino-terminal end. This region contains the active Tyr-783 and is homologous to the amino-terminal region of the DNA-binding bacterial gyrase GyrA subunit, suggesting a conserved DNA-binding mechanism.




FEBS letters


Hung F,Luo D,Sauvé DM,Muller MT,Roberge M




Has Abstract


1996-02-12 00:00:00














  • The expression of the H19 gene and its function in human bladder carcinoma cell lines.

    abstract::The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

    更新日期:1999-07-02 00:00:00

  • Aminoacyl-coenzyme A synthesis catalyzed by a CoA ligase from Penicillium chrysogenum.

    abstract::Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

    更新日期:2011-03-23 00:00:00

  • Molecular and functional characterization of a rat brain Kv beta 3 potassium channel subunit.

    abstract::A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heinemann SH,Rettig J,Wunder F,Pongs O

    更新日期:1995-12-27 00:00:00

  • Gene trap: a way to identify novel genes and unravel their biological function.

    abstract::The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Cecconi F,Meyer BI

    更新日期:2000-08-25 00:00:00

  • Bacterial beta-galactosidase and human dystrophin genes are expressed in mouse skeletal muscle fibers after ballistic transfection.

    abstract::Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

    更新日期:1997-09-08 00:00:00

  • Casein-derived bioactive phosphopeptides: role of phosphorylation and primary structure in promoting calcium uptake by HT-29 tumor cells.

    abstract::Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ferraretto A,Gravaghi C,Fiorilli A,Tettamanti G

    更新日期:2003-09-11 00:00:00

  • Different epitopes are required for gp130 activation by interleukin-6, oncostatin M and leukemia inhibitory factor.

    abstract::Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Timmermann A,Pflanz S,Grötzinger J,Küster A,Kurth I,Pitard V,Heinrich PC,Müller-Newen G

    更新日期:2000-02-25 00:00:00

  • Apicomplexan parasites contain a single lipoic acid synthase located in the plastid.

    abstract::Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomsen-Zieger N,Schachtner J,Seeber F

    更新日期:2003-07-17 00:00:00

  • pH dependence studies provide insight into the structure and mechanism of thimet oligopeptidase (EC

    abstract::Thimet oligopeptidase (EC; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

    更新日期:2003-06-19 00:00:00

  • Concomitant activation of Gi and Gq protein-coupled receptors does not require an increase in cytosolic calcium for platelet aggregation.

    abstract::U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pulcinelli FM,Pesciotti M,Pignatelli P,Riondino S,Gazzaniga PP

    更新日期:1998-09-11 00:00:00

  • Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

    abstract::Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

    更新日期:2018-04-01 00:00:00

  • Changes in L-ornithine decarboxylase activity in regenerating lung lobes.

    abstract::Growth of regenerating lung lobes is preceded by an increase of the activity of L-ornithine decarboxylase (ODC). Mechanical factors play a role in determining both the growth rate and enhancement of activity of ODC in regenerating lung lobes. Hormonal regulation also appears of importance as shown by the increased ODC...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tatár-Kiss S,Bardócz S,Kertai P

    更新日期:1984-09-17 00:00:00

  • Keratin 9 point mutation in the pedigree of epidermolytic hereditary palmoplantar keratoderma perturbs keratin intermediate filament network formation.

    abstract::Keratins form an intracellular keratin filament network in keratinocytes. Point mutations in the epidermal keratins could lead to the disruption of keratin filament formation, developing skin diseases such as epidermolytic hereditary palmoplantar keratoderma (EHPPK). We found a G to A transition in keratin 9 (K9) cDNA...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kobayashi S,Tanaka T,Matsuyoshi N,Imamura S

    更新日期:1996-05-20 00:00:00

  • Misacylation of pyrrolysine tRNA in vitro and in vivo.

    abstract::Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gundllapalli S,Ambrogelly A,Umehara T,Li D,Polycarpo C,Söll D

    更新日期:2008-10-15 00:00:00

  • Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.

    abstract::The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the pa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lamble HJ,Theodossis A,Milburn CC,Taylor GL,Bull SD,Hough DW,Danson MJ

    更新日期:2005-12-19 00:00:00

  • Channel-like NH3 flux by ammonium transporter AtAMT2.

    abstract::Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Neuhäuser B,Dynowski M,Ludewig U

    更新日期:2009-09-03 00:00:00

  • Unusual 5 S ribosomal RNAs. An analysis of individual segments can reveal phylogenetic relatedness.

    abstract::Sequence comparisons of 5 S and other ribosomal RNAs by segments can be useful in understanding anomalous primary and secondary structures and in assessing phylogenetic relationships. In a segmented analysis, the 5'-half of the Chlamydomonas reinhardii chloroplast 5 S ribosomal RNA is found to have a very close sequen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Delihas N

    更新日期:1987-09-14 00:00:00

  • Occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse.

    abstract::Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiorilli A,Siniscalco C,Chiarini A,Di Francesco L,Venerando B,Tettamanti G

    更新日期:1991-05-06 00:00:00

  • Identification and characterization of a novel Delta6-fatty acid desaturase gene from Rhizopus arrhizus.

    abstract::A cDNA sequence putatively encoding a Delta(6)-fatty acid desaturase was isolated from Rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends methods. Sequence analysis indicated that this cDNA sequence had an open reading frame of 1377 bp encoding 458 amino acids ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang Q,Li M,Ma H,Sun Y,Xing L

    更新日期:2004-01-02 00:00:00

  • Apolipoprotein E protects astrocytes from hypoxia and glutamate-induced apoptosis.

    abstract::Apolipoprotein E (apoE) is predominantly synthesized by astrocytes in the brain. In this study, we investigated the role of apoE in astrocyte apoptosis. We demonstrated that apoE protects astrocytes from hypoxia-induced apoptosis in a dose-dependent manner. Glutamate release from astrocytic cultures is significantly l...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhou S,Wu H,Zeng C,Xiong X,Tang S,Tang Z,Sun X

    更新日期:2013-01-16 00:00:00

  • Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)?

    abstract::Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomson AJ

    更新日期:1991-07-22 00:00:00

  • Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.

    abstract::Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

    更新日期:2017-09-01 00:00:00

  • Tubulin and microtubule are potential targets for brain hexokinase binding.

    abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wágner G,Kovács J,Löw P,Orosz F,Ovádi J

    更新日期:2001-11-30 00:00:00

  • The interaction of amino-deuteromethylated melittin with phospholipid membranes studied by deuterium NMR.

    abstract::Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dempsey CE,Cryer GD,Watts A

    更新日期:1987-06-22 00:00:00

  • Enhanced levels of cathepsin B mRNA in murine tumors.

    abstract::Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two specie...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moin K,Rozhin J,McKernan TB,Sanders VJ,Fong D,Honn KV,Sloane BF

    更新日期:1989-02-13 00:00:00

  • SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges.

    abstract::SP-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. It contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. Evidence is presented that all the cysteine residues are i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kirszbaum L,Bozas SE,Walker ID

    更新日期:1992-02-03 00:00:00

  • Breakdown and synthesis of polyphosphoinositides in fMetLeuPhe-stimulated neutrophils.

    abstract::The interconversions of the inositol-containing lipids (PI, PI-P and PI-P2) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cockcroft S,Barrowman MM,Gomperts BD

    更新日期:1985-02-25 00:00:00

  • Activation of bile salt dependent lipase by (lyso)phosphatidic acid and platelet activating factor.

    abstract::The activity of breast milk BSDL was assayed with or without phospholipids as extra-intestinal effector candidates. Phosphatidic acid, lysophosphatidic acid and platelet activating factor but not phosphatidylcholine and lysophosphatidylcholine stimulated BSDL activity at least as efficiently as taurocholate. The appar...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fontbonne H,Puigserver A,Bouza B,Lombardo D,Ajandouz el H

    更新日期:2013-09-17 00:00:00

  • Skeletal muscle UCP2 and UCP3 gene expression in a rat cancer cachexia model.

    abstract::Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

    更新日期:1998-10-09 00:00:00

  • Biotin synthase mechanism: on the origin of sulphur.

    abstract::Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

    更新日期:1998-11-27 00:00:00