Characterization of topoisomerase II-DNA interaction and identification of a DNA-binding domain by ultraviolet laser crosslinking.


:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1.2 +/- 0.5 x 10(8) M(-1) was determined from kinetic analysis. Topoisomerase II showed highest affinity for supercoiled DNA. Limited proteolysis of crosslinked topoisomerase II-DNA complexes showed a site of crosslinking to be within a 29-kDa fragment with Leu-681 at its amino-terminal end. This region contains the active Tyr-783 and is homologous to the amino-terminal region of the DNA-binding bacterial gyrase GyrA subunit, suggesting a conserved DNA-binding mechanism.




FEBS letters


Hung F,Luo D,Sauvé DM,Muller MT,Roberge M




Has Abstract


1996-02-12 00:00:00














  • Characterization of human presenilin 1 using N-terminal specific monoclonal antibodies: Evidence that Alzheimer mutations affect proteolytic processing.

    abstract::The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mercken M,Takahashi H,Honda T,Sato K,Murayama M,Nakazato Y,Noguchi K,Imahori K,Takashima A

    更新日期:1996-07-08 00:00:00

  • Epithelial CaSR deficiency alters intestinal integrity and promotes proinflammatory immune responses.

    abstract::The intestinal epithelium is equipped with sensing receptor mechanisms that interact with luminal microorganisms and nutrients to regulate barrier function and gut immune responses, thereby maintaining intestinal homeostasis. Herein, we clarify the role of the extracellular calcium-sensing receptor (CaSR) using intest...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cheng SX,Lightfoot YL,Yang T,Zadeh M,Tang L,Sahay B,Wang GP,Owen JL,Mohamadzadeh M

    更新日期:2014-11-17 00:00:00

  • Assignment of the Fe-N epsilon (His) stretching mode in the resonance Raman spectra of a monomeric insect cyanomethaemoglobin.

    abstract::Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kerr EA,Yu NT,Gersonde K

    更新日期:1984-12-03 00:00:00

  • Zwitterionic amphiphiles that raise the bilayer to hexagonal phase transition temperature inhibit protein kinase C. The exception that proves the rule.

    abstract::We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Epand RM,Stafford A,Wang J,Epand RF

    更新日期:1992-06-15 00:00:00

  • Interaction of mutagenic tryptophan pyrolysate with DNA. CD spectral study on the binding specificity.

    abstract::The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Inohara T,Tarui M,Doi M,Inoue M,Ishida T

    更新日期:1993-06-21 00:00:00

  • Regulation of human growth and differentiation factor 3 gene expression by NANOG in human embryonic carcinoma NCCIT cells.

    abstract::We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park SW,Lim HY,Do HJ,Sung B,Huh SH,Uhm SJ,Song H,Chung HJ,Kim JH,Kim NH,Kim JH

    更新日期:2012-09-21 00:00:00

  • Primary structure of the alpha-subunit of vacuolar-type Na(+)-ATPase in Enterococcus hirae. Amplification of a 1000-bp fragment by polymerase chain reaction.

    abstract::A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kakinuma Y,Igarashi K,Konishi K,Yamato I

    更新日期:1991-11-04 00:00:00

  • Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis.

    abstract::The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wilmotte A,Van der Auwera G,De Wachter R

    更新日期:1993-02-08 00:00:00

  • Potato tuber phospholipids contain colneleic acid in the 2-position.

    abstract::Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fauconnier ML,Williams TD,Marlier M,Welti R

    更新日期:2003-03-13 00:00:00

  • FixL-like sensor FlbS of Brucella abortus binds haem and is necessary for survival within eukaryotic cells.

    abstract::Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roset MS,Almirón MA

    更新日期:2013-09-17 00:00:00

  • Potentiation of the antagonistic effect of ACTH11-24 on steroidogenesis by synthesis of covalent dimeric conjugates.

    abstract::Covalent dimerization of the adrenocorticotropin fragment ACTH11-24 increases its antagonistic potency on the ACTH-induced steroidogenesis in isolated bovine fasciculata/reticularis cells by 3 orders of magnitude when the C-termini are linked via a 10 A long spacer. This strong potentiation, probably mediated by cross...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fauchère JL,Rossier M,Capponi A,Vallotton MB

    更新日期:1985-04-22 00:00:00

  • Evidence from peptide mapping for a human renin zymogen.

    abstract::Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McIntyre GD,Chaudhuri S,Coggins JR

    更新日期:1984-10-01 00:00:00

  • Molecular evolution of the calmodulin gene.

    abstract::On the basis of the intron/exon organization and the intramolecular homology of DNA sequences, I propose a novel model for genesis of the calmodulin gene. A primordial calmodulin gene consisting of 51 base pairs (17 amino acids) was subjected to three-fold duplication to create modern calmodulin with four calcium-bind...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nojima H

    更新日期:1987-06-15 00:00:00

  • Tomosyn guides SNARE complex formation in coordination with Munc18 and Munc13.

    abstract::As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Li Y,Wang S,Li T,Zhu L,Ma C

    更新日期:2018-04-01 00:00:00

  • ATP-dependent degradation of SulA, a cell division inhibitor, by the HslVU protease in Escherichia coli.

    abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seong IS,Oh JY,Yoo SJ,Seol JH,Chung CH

    更新日期:1999-07-30 00:00:00

  • Insulin-induced translocation of glucose transporters in rat hindlimb muscles.

    abstract::Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klip A,Ramlal T,Young DA,Holloszy JO

    更新日期:1987-11-16 00:00:00

  • Inhibition of voltage-gated cationic channels in rat embryonic hypothalamic neurones and C1300 neuroblastoma cells by triphenylethylene antioestrogens.

    abstract::The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hardy SP,deFelipe C,Valverde MA

    更新日期:1998-09-04 00:00:00

  • Tumor promoter 12-O-tetradecanoylphorbol 13-acetate inhibits mas/angiotensin receptor-stimulated inositol phosphate production and intracellular Ca2+ elevation in the 401L-C3 neuronal cell line.

    abstract::Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jackson TR,Hanley MR

    更新日期:1989-07-17 00:00:00

  • Structural modeling of dual-affinity purified Pho84 phosphate transporter.

    abstract::The phosphate transporter Pho84 of Saccharomyces cerevisiae is predicted to contain 12 transmembrane (TM) regions, divided into two partially duplicated parts of 6 TM segments. The three-dimensional (3D) organization of the Pho84 protein has not yet been determined. However, the 3D crystal structure of the Escherichia...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lagerstedt JO,Voss JC,Wieslander A,Persson BL

    更新日期:2004-12-17 00:00:00

  • The hinge region of chicken annexin I contains no site for tyrosine phosphorylation.

    abstract::Annexin I (AnxI) is a calcium-dependent membrane binding protein which has been implicated in various physiological activities. The region of the chicken anxI cDNA encoding the first 130 amino terminal residues was cloned by reverse transcription PCR in order to determine the relationship of its variable amino-termina...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sidis Y,Horseman ND

    更新日期:1993-08-30 00:00:00

  • The respiration-driven active sodium transport system in E. coli does not function with lithium.

    abstract::Comparison of respiration-driven active transport of alkali cations from E. coli cells loaded with Na+ or Li+ showed that Li+ could not be expelled from the cells like Na+. K+ accumulation, which was fast in Na+-loaded cells, was strongly inhibited in Li+-loaded cells, despite high membrane potential and respiratory r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Verkhovskaya ML,Verkhovsky MI,Wikström M

    更新日期:1996-06-17 00:00:00

  • Regulation of acetylcholine receptor gene expression in rats treated with alpha-bungarotoxin.

    abstract::Regulation of acetylcholine receptor (AChR) gene expression was analyzed in alpha-bungarotoxin (alpha-BTX) treated rats. A reduction in available 125I-alpha-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR alpha-, beta- epsilon- and delta-subunit mRNAs was simi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Asher O,Provenzano C,Fuchs S

    更新日期:1991-05-06 00:00:00

  • Antioxidant properties of omeprazole.

    abstract::Potential antioxidant properties of therapeutically achievable concentrations of the protonated, active form of omeprazole (OM) were investigated in vitro at specific acidic pH values to mimic intragastric conditions in the clinical setting. We found that OM is a powerful scavenger of hypochlorous acid (HOCl) even at ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lapenna D,de Gioia S,Ciofani G,Festi D,Cuccurullo F

    更新日期:1996-03-11 00:00:00

  • SKK4, a novel activator of stress-activated protein kinase-1 (SAPK1/JNK).

    abstract::A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38beta, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homolog...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lawler S,Cuenda A,Goedert M,Cohen P

    更新日期:1997-09-01 00:00:00

  • Identification of Lys190 as the primary binding site for pyridoxal 5'-phosphate in human serum albumin.

    abstract::The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bohney JP,Fonda ML,Feldhoff RC

    更新日期:1992-02-24 00:00:00

  • TNF-alpha enhances intracellular glucocorticoid availability.

    abstract::For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heiniger CD,Rochat MK,Frey FJ,Frey BM

    更新日期:2001-11-02 00:00:00

  • Regulation of the unfolded protein response in yeast by oxidative stress.

    abstract::In the unfolded protein response (UPR), Ire1 activates Hac1 to coordinate the transcription of hundreds of genes to mitigate ER stress. Recent work in Caenorhabditis elegans suggests that oxidative stress inhibits this canonical Ire1 signalling pathway, activating instead an antioxidant stress response. We sought to d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guerra-Moreno A,Ang J,Welsch H,Jochem M,Hanna J

    更新日期:2019-05-01 00:00:00

  • Modulation of intracellular Ca2+ in the parathyroid cell. Release of Ca2+ from non-mitochondrial pools by inositol trisphosphate.

    abstract::Stimuli which enhance secretion from parathyroid cells such as low extracellular Ca2+ or Mg2+ are associated with a decrease in the cytosolic Ca2+ concentration as measured by quin2. Current evidence suggests that increased production of inositol 1,4,5-triphosphate (IP3) releases Ca2+ from cellular stores thus increas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Epstein PA,Prentki M,Attie MF

    更新日期:1985-08-19 00:00:00

  • In vitro reconstitution of messenger ribonucleoprotein particles from globin messenger RNA and cytosol proteins.

    abstract::Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaedigk R,Oehler S,Köhler K,Setyono B

    更新日期:1985-01-07 00:00:00

  • Inhibition of lipid peroxidation by ubiquinol in submitochondrial particles in the absence of vitamin E.

    abstract::The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

    更新日期:1991-07-08 00:00:00