Characterization of topoisomerase II-DNA interaction and identification of a DNA-binding domain by ultraviolet laser crosslinking.

Abstract:

:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1.2 +/- 0.5 x 10(8) M(-1) was determined from kinetic analysis. Topoisomerase II showed highest affinity for supercoiled DNA. Limited proteolysis of crosslinked topoisomerase II-DNA complexes showed a site of crosslinking to be within a 29-kDa fragment with Leu-681 at its amino-terminal end. This region contains the active Tyr-783 and is homologous to the amino-terminal region of the DNA-binding bacterial gyrase GyrA subunit, suggesting a conserved DNA-binding mechanism.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

doi

10.1016/0014-5793(96)00035-x

subject

Has Abstract

pub_date

1996-02-12 00:00:00

pages

127-32

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(96)00035-X

journal_volume

380

pub_type

杂志文章
  • Peroxynitrite causes calcium efflux from mitochondria which is prevented by Cyclosporin A.

    abstract::Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00461-7

    authors: Packer MA,Murphy MP

    更新日期:1994-05-30 00:00:00

  • A delayed rectifier potassium channel cloned from bovine adrenal medulla. Functional analysis after expression in Xenopus oocytes and in a neuroblastoma cell line.

    abstract::Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Ne...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)01117-6

    authors: García-Guzmán M,Sala F,Criado M,Sala S

    更新日期:1994-11-07 00:00:00

  • 2-Cyclopenten-1-one and prostaglandin J2 reduce restenosis after balloon angioplasty in rats: role of NF-kappaB.

    abstract::The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(03)00873-1

    authors: Ianaro A,Maffia P,Cuzzocrea S,Mazzon E,Santoro MG,Di Rosa M,Ialenti A

    更新日期:2003-10-09 00:00:00

  • Identification of a 42 kDa protein as a substrate of protein phosphatase 1 in cilia from Paramecium.

    abstract::Okadaic acid, a specific inhibitor of protein phosphatase 1 in Paramecium causes sustained backward swimming in response to depolarising stimuli (S. Klumpp et al. (1990) EMBO J. 9, 685). Here, we employ okadaic acid, tautomycin, microcystin LR and inhibitor 1 as phosphatase inhibitors to identify a 42 kDa protein in t...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)81003-q

    authors: Klumpp S,Schultz JE

    更新日期:1991-08-19 00:00:00

  • A role for glycosaminoglycans in the development of collagen fibrils.

    abstract::Extensive data on the glycosaminoglycan (GAG) composition and the collagen fibril diameter distribution have been collected for a diverse range of connective tissues. It is shown that tissues with the smallest diameter collagen fibrils (mass-average diameter less than 60 nm) have high concentrations of hyaluronic acid...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/0014-5793(82)81060-0

    authors: Parry DA,Flint MH,Gillard GC,Craig AS

    更新日期:1982-11-22 00:00:00

  • Polynomial algebra reveals diverging roles of the unfolded protein response in endothelial cells during ischemia-reperfusion injury.

    abstract::The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2014.05.065

    authors: Le Pape S,Dimitrova E,Hannaert P,Konovalov A,Volmer R,Ron D,Thuillier R,Hauet T

    更新日期:2014-08-25 00:00:00

  • 5-Azacytidine increases the synthesis of embryonic hemoglobin (E2) in murine erythroleukemic cells.

    abstract::The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80803-5

    authors: Zucker RM,Decal DL,Whittington KB

    更新日期:1983-10-17 00:00:00

  • Geometry of binding of the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino phenylalanine to thrombin and trypsin. X-ray crystal structures of their trypsin complexes and modeling of their thrombin complexes.

    abstract::The X-ray crystal structures of the complexes formed with bovine trypsin and the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino-D,L-phenylalanine (3-TAPAP, 4-TAPAP and 4-TGPAP) were determined with data to 1.8 A resolution. The L-stereoisomer of 3-TAPAP binds as a compact entity into the activ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80033-y

    authors: Turk D,Stürzebecher J,Bode W

    更新日期:1991-08-05 00:00:00

  • Identification of a cysteine involved in the interaction between carbon monoxide dehydrogenase and corrinoid/Fe-S protein from Clostridium thermoaceticum.

    abstract::In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81808-d

    authors: Shanmugasundaram T,Sundaresh CS,Kumar GK

    更新日期:1993-07-12 00:00:00

  • Molecular cloning, characterization and evolution of the gene encoding a new group of short-chain alpha-neurotoxins in an Australian elapid, Pseudonaja textilis.

    abstract::The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain alpha-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a res...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)01549-0

    authors: Gong N,Armugam A,Jeyaseelan K

    更新日期:2000-05-19 00:00:00

  • cDNA encoding the chicken ortholog of the mouse dilute gene product. Sequence comparison reveals a myosin I subfamily with conserved C-terminal domains.

    abstract::We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)81123-4

    authors: Sanders G,Lichte B,Meyer HE,Kilimann MW

    更新日期:1992-10-26 00:00:00

  • Evolution of dnaQ, the gene encoding the editing 3' to 5' exonuclease subunit of DNA polymerase III holoenzyme in Gram-negative bacteria.

    abstract::The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(96)01361-0

    authors: Huang Y,Braithwaite DK,Ito J

    更新日期:1997-01-02 00:00:00

  • Altered lysosome distribution is an early neuropathological event in neurological forms of Gaucher disease.

    abstract::In the lysosomal storage disorder Gaucher disease (GD), glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of GD patients develops neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which ar...

    journal_title:FEBS letters

    pub_type: 信件

    doi:10.1002/1873-3468.12591

    authors: Zigdon H,Meshcheriakova A,Farfel-Becker T,Volpert G,Sabanay H,Futerman AH

    更新日期:2017-03-01 00:00:00

  • A novel peptidyl-prolyl cis/trans isomerase from Escherichia coli.

    abstract::A novel peptidyl-prolyl cis/trans isomerase was isolated from Escherichia coli cell extract and characterized partially. Determination of the molecular mass by electrospray mass spectrometry indicated a protein of 10102 +/- 2 Da, smaller than cyclophilins or FK 506 binding proteins currently known. The specificity con...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)80608-x

    authors: Rahfeld JU,Schierhorn A,Mann K,Fischer G

    更新日期:1994-04-18 00:00:00

  • Protective effect of L-carnitine on hyperammonemia.

    abstract::Inborn errors of the urea cycle, liver malfunction and drug-induced hepatotoxicity are causes of life-threatening encephalopathies arising from hyperammonemia. L-Carnitine prevented entirely ammonia toxicity in mice when injected intraperitoneally 30 min before a lethal dose of ammonium acetate. Survival depends on th...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80106-4

    authors: O'Connor JE,Costell M,Grisolía S

    更新日期:1984-01-30 00:00:00

  • Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates.

    abstract::The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had bee...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80408-2

    authors: Schmid FX

    更新日期:1986-03-31 00:00:00

  • A plasma-membrane progesterone receptor in human sperm is switched on by increasing intracellular free calcium.

    abstract::Recent studies have revealed the presence of an atypical progesterone receptor on the plasma membrane of a relatively small subpopulation of human sperm cells. In this study, we show that the receptor is present in many cells in a latent form and that the receptor function in these cells is switched on asynchronously ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80919-l

    authors: Mendoza C,Tesarik J

    更新日期:1993-09-06 00:00:00

  • Discrimination between the activity of protein kinase CK2 holoenzyme and its catalytic subunits.

    abstract::The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.06.031

    authors: Salvi M,Sarno S,Marin O,Meggio F,Itarte E,Pinna LA

    更新日期:2006-07-10 00:00:00

  • Evidence for a novel phosphopantetheinyl transferase domain in the polyketide synthase for enediyne biosynthesis.

    abstract::The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.02.061

    authors: Murugan E,Liang ZX

    更新日期:2008-04-02 00:00:00

  • MECP2 mutation in male patients with non-specific X-linked mental retardation.

    abstract::In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)01994-3

    authors: Orrico A,Lam C,Galli L,Dotti MT,Hayek G,Tong SF,Poon PM,Zappella M,Federico A,Sorrentino V

    更新日期:2000-09-22 00:00:00

  • An element of symmetry in yeast TATA-box binding protein transcription factor IID. Consequence of an ancestral duplication?

    abstract::TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/0014-5793(90)80558-z

    authors: Stucka R,Feldmann H

    更新日期:1990-02-26 00:00:00

  • Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor.

    abstract::Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80131-l

    authors: Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

    更新日期:1991-01-28 00:00:00

  • A high resolution 1H NMR study of the solution structure of human epidermal growth factor.

    abstract::500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80869-9

    authors: Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

    更新日期:1986-09-01 00:00:00

  • Multiple genes for G protein-coupled receptors and their expression in lingual epithelia.

    abstract::Using the polymerase chain reaction (PCR), we identified a gene family including more than 60 members which encoded similar G protein-coupled seven-transmembrane receptors. Sequence analyses of six representatives out of the 60 PCR clones showed that they had significant structural similarity to olfactory and optic re...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81302-g

    authors: Abe K,Kusakabe Y,Tanemura K,Emori Y,Arai S

    更新日期:1993-02-01 00:00:00

  • Cyclic AMP raises cytosolic Ca2+ and promotes Ca2+ influx in a clonal pancreatic beta-cell line (HIT T-15).

    abstract::The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80884-0

    authors: Prentki M,Glennon MC,Geschwind JF,Matschinsky FM,Corkey BE

    更新日期:1987-08-10 00:00:00

  • The RacGAP protein FilGAP is a negative regulator of chemokine-promoted lymphocyte migration.

    abstract::Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...

    journal_title:FEBS letters

    pub_type: 信件

    doi:10.1002/1873-3468.12189

    authors: Iida T,Saito K,Katagiri K,Kinashi T,Ohta Y

    更新日期:2016-05-01 00:00:00

  • A novel positive tetracycline-dependent transactivator (rtTA) variant with reduced background activity and enhanced activation potential.

    abstract::The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and m...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)02587-5

    authors: Kämper MR,Gohla G,Schlüter G

    更新日期:2002-04-24 00:00:00

  • A phosphorothioate oligonucleotide blocks reverse transcription via an antisense mechanism.

    abstract::We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (< 100 nM), the cleavage of the RNA template by the retroviral RNase H at the ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)80145-2

    authors: Boiziau C,Moreau S,Toulmé JJ

    更新日期:1994-03-07 00:00:00

  • Amplification and expression of recombinant genes in serum-independent Chinese hamster ovary cells.

    abstract::CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01328-8

    authors: Gandor C,Leist C,Fiechter A,Asselbergs FA

    更新日期:1995-12-27 00:00:00

  • Gibberellin biosynthesis in bacteria: separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum.

    abstract::Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional di...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.12.052

    authors: Morrone D,Chambers J,Lowry L,Kim G,Anterola A,Bender K,Peters RJ

    更新日期:2009-01-22 00:00:00