Abstract:
:Type II restriction endonucleases (REases) exist in multiple oligomeric forms. The tetrameric REases have two DNA binding interfaces and must synapse two recognition sites to achieve cleavage. It was hypothesised that binding of two recognition sites by tetrameric enzymes contributes to their fidelity. Here, we experimentally determined the fidelity for Bse634I REase in different oligomeric states. Surprisingly, we find that tetramerisation does not increase REase fidelity in comparison to the dimeric variant. Instead, an inherent ability to act concertedly at two sites provides tetrameric REase with a safety-catch to prevent host DNA cleavage if a single unmodified site becomes available.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zaremba M,Sasnauskas G,Siksnys Vdoi
10.1016/j.febslet.2012.07.009subject
Has Abstractpub_date
2012-09-21 00:00:00pages
3324-9issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00577-7journal_volume
586pub_type
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