Abstract:
:Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-Melittin at 4 mol% (peptide:lipid) induced reversible transitions between extended bilayers and micelles at the phase-transition temperature in dimyristoylphosphatidylcholine (DMPC) bilayers. These changes in lipid morphology did not occur at 1 mol% D-melittin: DMPC and the peptide was highly motionally restricted in gel in gel-phase lipid.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dempsey CE,Cryer GD,Watts Adoi
10.1016/0014-5793(87)81041-4subject
Has Abstractpub_date
1987-06-22 00:00:00pages
173-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)81041-4journal_volume
218pub_type
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