Involvement of decreased myo-inositol transport in lipopolysaccharide-induced depression of phosphoinositide hydrolysis in vascular smooth muscle.


:The mechanism underlying lipopolysaccharide (LPS)-induced depression of phosphoinositide (PI) hydrolysis was investigated using rat aortas. In LPS-pretreated aortas, the 5-hydroxytryptamine-stimulated accumulation of inositol monophosphate and incorporation of exogenous myo-inositol into PIs were significantly less than those in control aortas. Both sodium-myo-inositol cotransporter (SMIT) and phosphatidylinositol transfer protein (PITP) genes were constituently expressed in rat aortas. The mRNA level of SMIT was remarkably lower in LPS-pretreated aortas, while that of PITP mRNA was not affected by LPS. These results suggest that LPS-induced depression of SMIT expression is involved in inhibition of agonist-stimulated PI hydrolysis by LPS.




FEBS letters


Sotoda Y,Negoro M,Wakabayashi I





Has Abstract


2002-05-22 00:00:00














  • Mitochondrial uncoupling protein 2 gene transcript levels are elevated in maturating erythroid cells.

    abstract::Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

    更新日期:2007-03-20 00:00:00

  • Interaction between the delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus and liposomes.

    abstract::The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phospha...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yunovitz H,Yawetz A

    更新日期:1988-03-28 00:00:00

  • Replacement of Mn(III) with Cu(II) in Bacillus stearothermophilus superoxide dismutase. Similarity of the active site to the zinc site of copper/zinc superoxide dismutase.

    abstract::Copper(II) was substituted for manganese(III) in Bacillus stearothermophilus Mn-superoxide dismutase. The (EPR) spectrum of the Cu(II) is distinctly rhombic, overlapping that of Cu(II) replacing zinc in copper/zinc superoxide dismutase. The copper-cyanide complex of the bacterial enzyme gives an EPR spectrum nearly id...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bannister JV,Desideri A,Rotilio G

    更新日期:1985-08-19 00:00:00

  • Cardiolipin deficiency in Barth syndrome is not associated with increased superoxide/H2 O2 production in heart and skeletal muscle mitochondria.

    abstract::Barth syndrome (BTHS) is a rare X-linked genetic disorder caused by mutations in the gene encoding the transacylase tafazzin and characterized by loss of cardiolipin and severe cardiomyopathy. Mitochondrial oxidants have been implicated in the cardiomyopathy in BTHS. Eleven mitochondrial sites produce superoxide/hydro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Goncalves RLS,Schlame M,Bartelt A,Brand MD,Hotamışlıgil GS

    更新日期:2020-10-28 00:00:00

  • Susceptibility of protein kinase C to oxidative inactivation: loss of both phosphotransferase activity and phorbol diester binding.

    abstract::Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gopalakrishna R,Anderson WB

    更新日期:1987-12-10 00:00:00

  • Mitochondrial dynamics, mitophagy and biogenesis in neonatal hypoxic-ischaemic brain injury.

    abstract::Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

    更新日期:2018-03-01 00:00:00

  • Protein syntheis in brain mitochondrial and synaptosomal preparations.

    abstract::The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Morgan IG

    更新日期:1970-10-16 00:00:00

  • Acidic phospholipids directly inhibit DNA binding of mammalian DNA topoisomerase I.

    abstract::Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

    更新日期:1990-02-12 00:00:00

  • Peculiarities of immunoglobulin gene structures as a basis for somatic mutation emergence.

    abstract::Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kolchanov NA,Solovyov VV,Rogozin IB

    更新日期:1987-04-06 00:00:00

  • Primary structure of porcine muscarinic acetylcholine receptor III and antagonist binding studies.

    abstract::The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

    更新日期:1988-08-01 00:00:00

  • Upregulation of microRNA-107 induces proliferation in human gastric cancer cells by targeting the transcription factor FOXO1.

    abstract::MicroRNA-107 (miR-107) has been demonstrated to regulate proliferation and apoptosis in many types of cancers. Nevertheless, its biological function in gastric cancer remains largely unexplored. Here, we found that the expression level of miR-107 was increased in gastric cancer in comparison with the adjacent normal t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li F,Liu B,Gao Y,Liu Y,Xu Y,Tong W,Zhang A

    更新日期:2014-02-14 00:00:00

  • Cyclooxygenase-catalyzed formation of 9-hydroxylinoleic acid by guinea pig alveolar macrophages under non-stimulated conditions.

    abstract::Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Engels F,Willems H,Nijkamp FP

    更新日期:1986-12-15 00:00:00

  • Site-directed mutagenesis but not gamma-carboxylation of Glu-35 in factor VIIa affects the association with tissue factor.

    abstract::Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Persson E,Nielsen LS

    更新日期:1996-05-06 00:00:00

  • Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer.

    abstract::Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

    更新日期:1983-07-11 00:00:00

  • alpha-Allenyl putrescine, an enzyme-activated irreversible inhibitor of bacterial and mammalian ornithine decarboxylases.

    abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Danzin C,Casara P

    更新日期:1984-09-03 00:00:00

  • Synthesis of phage-specific transfer RNA molecules by vibriophage phi 149.

    abstract::32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ghosh RK,Guhathakurta I

    更新日期:1983-10-03 00:00:00

  • Phosphate regulation of biosynthesis of extracellular RNases of endospore-forming bacteria.

    abstract::The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

    更新日期:1995-01-02 00:00:00

  • Photosensitive liposomes as 'cages' for laser-triggered solute delivery: the effect of bilayer cholesterol on kinetics of solute release.

    abstract::Liposomes containing acyl chains incorporating azobenzene chromophores have been investigated as potential 'caging' agents for fast solute release. On photolysis, trapped marker dye can be released from gel-phase liposomes within milliseconds. Solute release is markedly sensitive to the presence of cholesterol in the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bisby RH,Mead C,Morgan CG

    更新日期:1999-12-10 00:00:00

  • Identification, characterization, and molecular cloning of a novel transporter-like protein localized to the central nervous system.

    abstract::During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

    更新日期:1992-11-09 00:00:00

  • Expression, purification and characterization of recombinant human proinsulin.

    abstract::We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cowley DJ,Mackin RB

    更新日期:1997-02-03 00:00:00

  • The roles of organic anion permeases in aluminium resistance and mineral nutrition.

    abstract::Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Delhaize E,Gruber BD,Ryan PR

    更新日期:2007-05-25 00:00:00

  • Metal regulation of metallothionein participation in redox reactions.

    abstract::Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang S,Li J,Wang CC,Tsou CL

    更新日期:1999-12-03 00:00:00

  • 4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2.

    abstract::In nucleus, eIF4E regulates the nucleus export of specific mRNA. In this study, altered 4E-BP3 (eIF4E-binding protein 3) expression resulted in profoundly affected cyclin D1 protein levels, partially due to changes in the cytoplasmic cyclin D1 mRNA levels in both U2OS and MCF7 cells, whereas altered 4E-BP1 expression ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen CC,Lee JC,Chang MC

    更新日期:2012-07-30 00:00:00

  • Functional properties of a pore mutant in the Drosophila melanogaster inositol 1,4,5-trisphosphate receptor.

    abstract::The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Srikanth S,Wang Z,Hasan G,Bezprozvanny I

    更新日期:2004-09-24 00:00:00

  • Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.

    abstract::Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist expos...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Goral V,Wu Q,Sun H,Fang Y

    更新日期:2011-04-06 00:00:00

  • Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2.

    abstract::A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berendes R,Burger A,Voges D,Demange P,Huber R

    更新日期:1993-02-08 00:00:00

  • Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length.

    abstract::Alzheimer's disease is characterized by the deposition of amyloid beta-peptide (Abeta) plaques in the brain. Full-length amyloid-beta precursor protein (APP) is processed by alpha- and beta-secretases to yield soluble APP derivatives and membrane-bound C-terminal fragments, which are further processed by gamma-secreta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lundin C,Johansson S,Johnson AE,Näslund J,von Heijne G,Nilsson I

    更新日期:2007-08-07 00:00:00

  • Gene therapy for streptozotocin-induced diabetic mice by electroporational transfer of naked human insulin precursor DNA into skeletal muscle in vivo.

    abstract::Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yin D,Tang JG

    更新日期:2001-04-20 00:00:00

  • Sodium nitroprusside inhibits proliferation and putrescine synthesis in human colon carcinoma cells.

    abstract::In human colon carcinoma HT-29 Glc(-/+) cells, L-arginine is the common precursor of polyamines which are absolutely necessary for cellular proliferation and nitric oxide (NO) with reported anti-proliferative activity. The aim of the present work was to test the effect of the NO donor sodium nitroprusside (SNP) on pol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Blachier F,Robert V,Selamnia M,Mayeur C,Duee PH

    更新日期:1996-11-04 00:00:00

  • Studies on the substrate for hepatic lipogenesis in the rat.

    abstract::The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Geelen MJ,Hindriks GA

    更新日期:1984-09-17 00:00:00