Involvement of decreased myo-inositol transport in lipopolysaccharide-induced depression of phosphoinositide hydrolysis in vascular smooth muscle.

abstract：:Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

journal_title：FEBS letters

authors： Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

更新日期：2007-03-20 00:00:00

abstract：:The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phospha...

journal_title：FEBS letters

authors： Yunovitz H,Yawetz A

更新日期：1988-03-28 00:00:00

abstract：:Copper(II) was substituted for manganese(III) in Bacillus stearothermophilus Mn-superoxide dismutase. The (EPR) spectrum of the Cu(II) is distinctly rhombic, overlapping that of Cu(II) replacing zinc in copper/zinc superoxide dismutase. The copper-cyanide complex of the bacterial enzyme gives an EPR spectrum nearly id...

journal_title：FEBS letters

authors： Bannister JV,Desideri A,Rotilio G

更新日期：1985-08-19 00:00:00

abstract：:Barth syndrome (BTHS) is a rare X-linked genetic disorder caused by mutations in the gene encoding the transacylase tafazzin and characterized by loss of cardiolipin and severe cardiomyopathy. Mitochondrial oxidants have been implicated in the cardiomyopathy in BTHS. Eleven mitochondrial sites produce superoxide/hydro...

journal_title：FEBS letters

authors： Goncalves RLS,Schlame M,Bartelt A,Brand MD,Hotamışlıgil GS

更新日期：2020-10-28 00:00:00

abstract：:Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...

journal_title：FEBS letters

authors： Gopalakrishna R,Anderson WB

更新日期：1987-12-10 00:00:00

abstract：:Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

journal_title：FEBS letters

authors： Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

更新日期：2018-03-01 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

journal_title：FEBS letters

authors： Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

更新日期：1990-02-12 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:MicroRNA-107 (miR-107) has been demonstrated to regulate proliferation and apoptosis in many types of cancers. Nevertheless, its biological function in gastric cancer remains largely unexplored. Here, we found that the expression level of miR-107 was increased in gastric cancer in comparison with the adjacent normal t...

journal_title：FEBS letters

authors： Li F,Liu B,Gao Y,Liu Y,Xu Y,Tong W,Zhang A

更新日期：2014-02-14 00:00:00

abstract：:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

journal_title：FEBS letters

authors： Engels F,Willems H,Nijkamp FP

更新日期：1986-12-15 00:00:00

abstract：:Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

journal_title：FEBS letters

authors： Persson E,Nielsen LS

更新日期：1996-05-06 00:00:00

abstract：:Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

journal_title：FEBS letters

authors： Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

更新日期：1983-07-11 00:00:00

abstract：:alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...

journal_title：FEBS letters

authors： Danzin C,Casara P

更新日期：1984-09-03 00:00:00

abstract：:32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

journal_title：FEBS letters

authors： Ghosh RK,Guhathakurta I

更新日期：1983-10-03 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:Liposomes containing acyl chains incorporating azobenzene chromophores have been investigated as potential 'caging' agents for fast solute release. On photolysis, trapped marker dye can be released from gel-phase liposomes within milliseconds. Solute release is markedly sensitive to the presence of cholesterol in the ...

journal_title：FEBS letters

authors： Bisby RH,Mead C,Morgan CG

更新日期：1999-12-10 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

journal_title：FEBS letters

authors： Delhaize E,Gruber BD,Ryan PR

更新日期：2007-05-25 00:00:00

abstract：:Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithio...

journal_title：FEBS letters

authors： Zhang S,Li J,Wang CC,Tsou CL

更新日期：1999-12-03 00:00:00

abstract：:In nucleus, eIF4E regulates the nucleus export of specific mRNA. In this study, altered 4E-BP3 (eIF4E-binding protein 3) expression resulted in profoundly affected cyclin D1 protein levels, partially due to changes in the cytoplasmic cyclin D1 mRNA levels in both U2OS and MCF7 cells, whereas altered 4E-BP1 expression ...

journal_title：FEBS letters

authors： Chen CC,Lee JC,Chang MC

更新日期：2012-07-30 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

journal_title：FEBS letters

authors： Srikanth S,Wang Z,Hasan G,Bezprozvanny I

更新日期：2004-09-24 00:00:00

abstract：:Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist expos...

journal_title：FEBS letters

authors： Goral V,Wu Q,Sun H,Fang Y

更新日期：2011-04-06 00:00:00

abstract：:A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influ...

journal_title：FEBS letters

authors： Berendes R,Burger A,Voges D,Demange P,Huber R

更新日期：1993-02-08 00:00:00

abstract：:Alzheimer's disease is characterized by the deposition of amyloid beta-peptide (Abeta) plaques in the brain. Full-length amyloid-beta precursor protein (APP) is processed by alpha- and beta-secretases to yield soluble APP derivatives and membrane-bound C-terminal fragments, which are further processed by gamma-secreta...

journal_title：FEBS letters

authors： Lundin C,Johansson S,Johnson AE,Näslund J,von Heijne G,Nilsson I

更新日期：2007-08-07 00:00:00

abstract：:Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

journal_title：FEBS letters

authors： Yin D,Tang JG

更新日期：2001-04-20 00:00:00

abstract：:In human colon carcinoma HT-29 Glc(-/+) cells, L-arginine is the common precursor of polyamines which are absolutely necessary for cellular proliferation and nitric oxide (NO) with reported anti-proliferative activity. The aim of the present work was to test the effect of the NO donor sodium nitroprusside (SNP) on pol...

journal_title：FEBS letters

authors： Blachier F,Robert V,Selamnia M,Mayeur C,Duee PH

更新日期：1996-11-04 00:00:00

abstract：:The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

journal_title：FEBS letters

authors： Geelen MJ,Hindriks GA

更新日期：1984-09-17 00:00:00