Abstract:
:cDNAs encoding rat adipose tissue hormone-sensitive lipase were expressed in COS cells, under the control of the SV40 promoter to half the level in rat adipocytes, the richest native source of the enzyme. A cDNA lacking most of the long 5'-untranslated region of the full-length rat hormone-sensitive lipase cDNA was, with regard to the lipase activity, on the average 70% more efficiently expressed that the full-length cDNA. The recombinant protein was almost identical to hormone-sensitive lipase of rat adipose tissue with respect to specific activity, susceptibility to inhibitors, molecular size, phosphorylation and activation by cyclic AMP-dependent protein kinase. The described eukaryotic expression system will allow analysis of effects of amino acid substitutions introduced into the lipase molecule by site-directed mutagenesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Holm C,Davis RC,Fredrikson G,Belfrage P,Schotz MCdoi
10.1016/0014-5793(91)80744-nkeywords:
subject
Has Abstractpub_date
1991-07-08 00:00:00pages
139-44issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80744-Njournal_volume
285pub_type
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