Abstract:
:Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mitochondria in the presence of a protonmotive force. Mitochondria incubated with NAD(P)H also presented intense O(2)(-) generation and NO degradation rates that were insensitive to respiratory inhibitors and abolished after proteinase treatment. These results suggest that an outer membrane-localized NAD(P)H oxidase activity, in addition to the electron leakage from the respiratory chain, promotes O(2)(-)-dependent NO degradation in rat liver mitochondria.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Oliveira HC,Saviani EE,Salgado Idoi
10.1016/j.febslet.2009.06.012subject
Has Abstractpub_date
2009-07-07 00:00:00pages
2276-80issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00469-4journal_volume
583pub_type
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