A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans.

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00

abstract：:The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...

journal_title：FEBS letters

authors： Bhattacharyya R,Wedegaertner PB

更新日期：2003-04-10 00:00:00

abstract：:The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...

journal_title：FEBS letters

authors： Arseniev AS,Barsukov IL,Bystrov VF,Lomize AL,Ovchinnikov YuA

更新日期：1985-07-08 00:00:00

abstract：:Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...

journal_title：FEBS letters

authors： Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl R

更新日期：1999-10-29 00:00:00

abstract：:The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...

journal_title：FEBS letters

authors： Ciruela F,McIlhinney RA

更新日期：1997-11-24 00:00:00

abstract：:Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of ...

journal_title：FEBS letters

authors： Loreni F,Amaldi F

更新日期：1997-10-27 00:00:00

abstract：:The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...

journal_title：FEBS letters

authors： Welsh JH,Zerbe O,von Philipsborn W,Robinson JA

更新日期：1992-02-10 00:00:00

abstract：:The pancreatic ATP-sensitive potassium channels comprise two subunits: SUR1 and Kir6.2. Two SUR1 mutations, A116P and V187D, reduce channel activity causing persistent hyperinsulinemic hypoglycemia of infancy. We investigated whether these mutations cause temperature sensitive misfolding. We show that the processing d...

journal_title：FEBS letters

authors： Yang K,Fang K,Fromondi L,Chan KW

更新日期：2005-08-01 00:00:00

abstract：:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

journal_title：FEBS letters

authors： Congote LF,Temmel N

更新日期：2004-10-22 00:00:00

abstract：:The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

journal_title：FEBS letters

authors： Geelen MJ,Hindriks GA

更新日期：1984-09-17 00:00:00

abstract：:In kidney cortex tubules isolated from fed rabbits L-alanine is not utilized as glucose precursor, when added as a sole substrate. However, this amino acid decreases gluconeogenesis from low (up to 1 mM) 2-oxoglutarate concentrations and stimulates this process at higher (2.5-10 mM) ketoacid contents in the suspension...

journal_title：FEBS letters

authors： Zabłocki K,Bryła J

更新日期：1989-12-18 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...

journal_title：FEBS letters

authors： Mauco G,Chap H,Douste-Blazy L

更新日期：1983-03-21 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the a...

journal_title：FEBS letters

authors： Nagahama M,Nakayama K,Hori H,Murakami K

更新日期：1989-12-18 00:00:00

abstract：:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...

journal_title：FEBS letters

authors： Sahin U,Blobel CP

更新日期：2007-01-09 00:00:00

abstract：:Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression...

journal_title：FEBS letters

authors： Park SW,Do HJ,Han MH,Choi W,Kim JH

更新日期：2018-01-01 00:00:00

abstract：:C2cd4c, encoded by a gene belonging to the C2cd4 family, contains a C2 domain conserved across species and is localized to the cytoplasm. To examine the role of C2cd4c in the pancreas, we studied its localization and generated C2cd4c knockout (KO) mice. C2cd4c was expressed in pancreatic endocrine progenitors at early...

journal_title：FEBS letters

authors： Omori H,Ogaki S,Sakano D,Sato M,Umeda K,Takeda N,Nakagata N,Kume S

更新日期：2016-08-01 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over...

journal_title：FEBS letters

authors： Mao XM,Zhou Z,Cheng LY,Hou XP,Guan WJ,Li YQ

更新日期：2009-10-06 00:00:00

abstract：:The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...

journal_title：FEBS letters

authors： Wang MH,Zhao KY

更新日期：1997-08-04 00:00:00

abstract：:Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of muta...

journal_title：FEBS letters

authors： Kazakova HA,Entelis NS,Martin RP,Tarassov IA

更新日期：1999-01-15 00:00:00

abstract：:Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...

journal_title：FEBS letters

authors： Bücking H,Förster H,Stenzel I,Miersch O,Hause B

更新日期：2004-03-26 00:00:00

abstract：:We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

journal_title：FEBS letters

authors： Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

更新日期：1997-07-14 00:00:00

abstract：:Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...

journal_title：FEBS letters

authors： Szanto A,Roszer T

更新日期：2008-01-09 00:00:00

abstract：:Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We conf...

journal_title：FEBS letters

authors： Xu C,Li Z,He H,Wernimont A,Li Y,Loppnau P,Min J

更新日期：2015-10-24 00:00:00

abstract：:The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition...

journal_title：FEBS letters

authors： Poirier H,Braissant O,Niot I,Wahli W,Besnard P

更新日期：1997-08-04 00:00:00