Abstract:
:Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase binding and surface plasmon resonance assays. These studies demonstrated a particularly high affinity (K(d) about 1 nM) of tropoelastin for microfibrillar fibulin-2 and the recently described nidogen-2 isoform. More moderate affinities were observed for fibulin-1, laminin-1 and perlecan, while several other ligands such as collagens, nidogen-1, fibronectin and BM-40 showed little or no binding. In immunogold staining of mouse aortic media, elastic fibers were heavily decorated with tropoelastin, fibulin-2 and nidogen-2, while the reaction with fibulin-1 was lower. The colocalization of these proteins emphasizes the potential for in vivo interactions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl Rdoi
10.1016/s0014-5793(99)01362-9keywords:
subject
Has Abstractpub_date
1999-10-29 00:00:00pages
280-4issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01362-9journal_volume
460pub_type
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