A simple method for determining kinetic constants of complexing inactivation at identical enzyme and inhibitor concentrations.

Abstract:

:The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting the derived equations to the progress curve. Numerical examples show that this method is valid and gives satisfactory results.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Wang MH,Zhao KY

doi

10.1016/s0014-5793(97)00771-0

subject

Has Abstract

pub_date

1997-08-04 00:00:00

pages

425-8

issue

3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(97)00771-0

journal_volume

412

pub_type

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