Crystal structure of human nuclear pore complex component NUP43.

abstract：:A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untransla...

journal_title：FEBS letters

authors： Kawamoto T,Mitsuuchi Y,Toda K,Miyahara K,Yokoyama Y,Nakao K,Hosoda K,Yamamoto Y,Imura H,Shizuta Y

更新日期：1990-09-03 00:00:00

abstract：:The inhibitor of gamma-glutamyl transpeptidase (gamma-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of gamma-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specif...

journal_title：FEBS letters

authors： Antczak C,Karp DR,London RE,Bauvois B

更新日期：2001-11-16 00:00:00

abstract：:Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...

journal_title：FEBS letters

authors： Fujisawa T,Kostyukova A,Maéda Y

更新日期：2001-06-01 00:00:00

abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...

journal_title：FEBS letters

authors： Hirata R,Ohsumi Y,Anraku Y

更新日期：1989-02-27 00:00:00

abstract：:The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...

journal_title：FEBS letters

authors： Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M

更新日期：2002-05-22 00:00:00

abstract：:The herpesvirus life cycle has two distinct phases: latency and lytic replication. The viral immediate early protein replication and transcription activator (RTA) plays a central role in mediating the balance between these two phases. Here, we demonstrate that a B cell terminal differentiation factor X-box binding pro...

journal_title：FEBS letters

authors： Yu F,Feng J,Harada JN,Chanda SK,Kenney SC,Sun R

更新日期：2007-07-24 00:00:00

abstract：:The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...

journal_title：FEBS letters

authors： Peri KG,MacKenzie RE

更新日期：1991-12-02 00:00:00

abstract：:Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...

journal_title：FEBS letters

authors： Burner U,Jantschko W,Obinger C

更新日期：1999-01-29 00:00:00

abstract：:In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...

journal_title：FEBS letters

authors： Peraldi P,Frödin M,Barnier JV,Calleja V,Scimeca JC,Filloux C,Calothy G,Van Obberghen E

更新日期：1995-01-09 00:00:00

abstract：:In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...

journal_title：FEBS letters

authors： Ji S,Beckh K,Jungermann K

更新日期：1984-02-13 00:00:00

abstract：:The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into...

journal_title：FEBS letters

authors： Salvi M,Sarno S,Marin O,Meggio F,Itarte E,Pinna LA

更新日期：2006-07-10 00:00:00

abstract：:The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

journal_title：FEBS letters

authors： Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

更新日期：2004-06-04 00:00:00

abstract：:Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...

journal_title：FEBS letters

authors： Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

更新日期：1995-07-03 00:00:00

abstract：:Skeletal muscle plays key roles in metabolic homeostasis. Loss of muscle mass, called muscle atrophy exacerbates disease-associated metabolic perturbations. In this study, we characterized the molecular functions and mechanisms underlying regulation of skeletal muscle atrophy induced by denervation. Denervation signif...

journal_title：FEBS letters

authors： Kang J,Jeong MG,Oh S,Jang EJ,Kim HK,Hwang ES

更新日期：2014-01-03 00:00:00

abstract：:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...

journal_title：FEBS letters

authors： Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

更新日期：1988-08-15 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very...

journal_title：FEBS letters

authors： Mohr E,Bahnsen U,Kiessling C,Richter D

更新日期：1988-12-19 00:00:00

abstract：:The major anthocyanin in A. thaliana is a cyanidin derivative modified by glycosylation as well as by the addition of three acyl moieties: malonyl, p-coumaroyl, and sinapoyl. We have isolated a member of the BAHD acyltransferase family which catalyzes this malonylation reaction by combining a reverse genetics approach...

journal_title：FEBS letters

authors： D'Auria JC,Reichelt M,Luck K,Svatos A,Gershenzon J

更新日期：2007-03-06 00:00:00

abstract：:Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

journal_title：FEBS letters

authors： Matsunami RK,Campion SR,Niyogi SK,Stevens A

更新日期：1990-05-07 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:[3H]inositol-labelled products are released from adrenal medullary cells during exocytotic secretion. In 'leaky' cells in which small molecules readily enter and leave the cytoplasm, addition of micromolar calcium ions in the presence of ATP stimulates exocytosis and causes the release of inositol polyphosphates. Thes...

journal_title：FEBS letters

authors： Whitaker M

更新日期：1985-09-09 00:00:00

abstract：:A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...

journal_title：FEBS letters

authors： Langbakk B,Flatmark T

更新日期：1984-09-03 00:00:00

abstract：:Amino acid sequences of cytotoxin-like basic proteins (CLBPs), purified from the venoms of Formosan cobra (Naja naja atra) and Indian cobra (Naja naja), were reinvestigated. The determined sequences differed from those reported previously by Takechi et al. [(1985) Biochem. Int. 11, 795-802; (1987) Biochem. Int. 14, 14...

journal_title：FEBS letters

authors： Inoue S,Okumura K,Tsujino M,Ohkura K,Ikeda K,Takechi M,Tanaka Y,Hayashi K

更新日期：1989-11-06 00:00:00

abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

journal_title：FEBS letters

authors： Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

更新日期：2007-02-20 00:00:00

abstract：:Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X ...

journal_title：FEBS letters

authors： Koshihara Y,Neichi T,Murota S,Lao A,Fujimoto Y,Tatsuno T

更新日期：1983-07-11 00:00:00

abstract：:Non-alcoholic fatty liver disease is tightly associated with insulin resistance, type 2 diabetes and obesity, but the molecular links between hepatic fat accumulation and insulin resistance are not fully identified. Excessive accumulation of triglycerides (TG) is one the main characteristics of non-alcoholic fatty liv...

journal_title：FEBS letters

authors： Denechaud PD,Dentin R,Girard J,Postic C

更新日期：2008-01-09 00:00:00

abstract：:C1qTNF-related proteins (CTRPs) are involved in diverse processes including metabolism, inflammation host defense, apoptosis, cell differentiation, autoimmunity, hibernation, and organogenesis. However, the physiological role of CTRP6 remains poorly understood. Here we demonstrate that the globular domain of CTRP6 med...

journal_title：FEBS letters

authors： Lee W,Kim MJ,Park EJ,Choi YJ,Park SY

更新日期：2010-03-05 00:00:00

abstract：:RNA molecules have been found to play important roles in DNA double-strand break (DSB) repair, but the exact underlying mechanism remains unclear. Here, we aimed to clarify the function of RNase L, an important ribonuclease in the immune system of vertebrates, in DSB repair. Knockdown of RNase L reduces cell survival ...

journal_title：FEBS letters

authors： Zhong Y,Pan B,Zhu J,Fu H,Zheng X

更新日期：2019-06-01 00:00:00

abstract：:Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...

journal_title：FEBS letters

authors： Vance JE

更新日期：2006-10-09 00:00:00

abstract：:A stabilization of both the precursor and the mature beta 1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt-4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differential effects of protein synthesis inhibitors are reported since the slow degradat...

journal_title：FEBS letters

authors： Vekeman S,Jaspers M,Cassiman JJ

更新日期：1993-07-26 00:00:00