Alpha 2 high molecular mass cysteine proteinase inhibitor: HMr alpha 2-CPI. An inhibitor of human liver cathepsin H as probed by kinetic study.

abstract：:Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxyg...

journal_title：FEBS letters

authors： Peer M,Bach M,Mueller MJ,Waller F

更新日期：2011-10-03 00:00:00

abstract：:Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperatur...

journal_title：FEBS letters

authors： Thomas K,Rutter A,Suller M,Harwood J,Lloyd D

更新日期：1998-03-20 00:00:00

abstract：:This study investigates to what extent the expression of the slow myosin heavy chain (MyHCI) isoform and the slow type sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) isoform are co-regulated in fibers of regenerating skeletal soleus muscle. Both overexpression of cain, a calcineurin inhibitor, or partial tenotomy preven...

journal_title：FEBS letters

authors： Zádor E,Fenyvesi R,Wuytack F

更新日期：2005-01-31 00:00:00

abstract：:Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...

journal_title：FEBS letters

authors： Singal B,Balakrishna AM,Nartey W,Manimekalai MSS,Jeyakanthan J,Grüber G

更新日期：2017-08-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...

journal_title：FEBS letters

authors： Boyne JR,Whitehouse A

更新日期：2009-11-19 00:00:00

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...

journal_title：FEBS letters

authors： Renneboog-Squilbin C

更新日期：1969-02-01 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced which reacts with human mitofilin, a mitochondrial inner membrane protein. This mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively. The first three are outer membrane proteins, CHC...

journal_title：FEBS letters

authors： Xie J,Marusich MF,Souda P,Whitelegge J,Capaldi RA

更新日期：2007-07-24 00:00:00

abstract：:Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, a...

journal_title：FEBS letters

authors： Mencarelli S,Cavalieri C,Magini A,Tancini B,Basso L,Lemansky P,Hasilik A,Li YT,Chigorno V,Orlacchio A,Emiliani C,Sonnino S

更新日期：2005-10-24 00:00:00

abstract：:We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

journal_title：FEBS letters

authors： Wang R,Kong C,Kolatkar P,Chung MC

更新日期：2001-11-23 00:00:00

abstract：:To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

journal_title：FEBS letters

authors： McKay MJ,Greathouse DV,Koeppe RE 2nd

更新日期：2020-12-01 00:00:00

abstract：:Selenium-dependent glutathione peroxidase activity is documented for the first time in insects. Reduction in glutathione peroxidase activity in the cytosol of adult house flies by lowering selenium in the diet results in significant increases in peroxidative injury. Catalase activity, while higher in low-selenium flie...

journal_title：FEBS letters

authors： Simmons TW,Jamall IS,Lockshin RA

更新日期：1987-06-29 00:00:00

abstract：:Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...

journal_title：FEBS letters

authors： Ballantine JE,Woodland HR

更新日期：1985-01-28 00:00:00

abstract：:The pancreatic ATP-sensitive potassium channels comprise two subunits: SUR1 and Kir6.2. Two SUR1 mutations, A116P and V187D, reduce channel activity causing persistent hyperinsulinemic hypoglycemia of infancy. We investigated whether these mutations cause temperature sensitive misfolding. We show that the processing d...

journal_title：FEBS letters

authors： Yang K,Fang K,Fromondi L,Chan KW

更新日期：2005-08-01 00:00:00

abstract：:Revised assignments for the bilayer helices of the beta-, gamma- and delta-subunits of the acetylcholine receptor are presented. A new feature of the model is extensive charge matching between the polar groups of the ion channel elements of different subunits. ...

journal_title：FEBS letters

authors： Kosower EM

更新日期：1984-06-25 00:00:00

abstract：:Pro-apoptotic functions of the BH3-only protein BAD are negatively regulated by survival signal-mediated phosphorylation at several serine residues. Recently, we found that the mutant BAD (BADD119G) with an amino acid substitution of Asp (Asp119 to Gly) within the BH3 domain displays strong pro-apoptotic activity in s...

journal_title：FEBS letters

authors： Adachi M,Zhang YB,Imai K

更新日期：2003-09-11 00:00:00

abstract：:The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...

journal_title：FEBS letters

authors： Guo W,Roth D,Gatti E,De Camilli P,Novick P

更新日期：1997-03-10 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...

journal_title：FEBS letters

authors： Miura D,Kida Y,Nojima H

更新日期：2007-05-29 00:00:00

abstract：:Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...

journal_title：FEBS letters

authors： Umetsu M,Tsumoto K,Ashish K,Nitta S,Tanaka Y,Adschiri T,Kumagai I

更新日期：2004-01-16 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:Escherichia coli maltose-binding protein (MBP) is frequently used as an affinity tag to facilitate the purification of recombinant proteins. An important additional attribute of MBP is its remarkable ability to enhance the solubility of its fusion partners. MBPs are present in a wide variety of microorganisms includin...

journal_title：FEBS letters

authors： Fox JD,Routzahn KM,Bucher MH,Waugh DS

更新日期：2003-02-27 00:00:00

abstract：:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...

journal_title：FEBS letters

authors： Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JK

更新日期：2001-12-07 00:00:00

abstract：:To evaluate the involvement of protein phosphatases (PP) in differentiation of human myelogenous leukemia HL-60 cells, we made use of potent inhibitors of PP1 and PP2A, calyculin-A (CAL-A) and okadaic acid (OKA). CAL-A and OKA could augment all-trans retinoic acid (ATRA)-induced granulocytic differentiation, whereas t...

journal_title：FEBS letters

authors： Morita K,Nishikawa M,Kobayashi K,Deguchi K,Ito M,Nakano T,Shima H,Nagao M,Kuno T,Tanaka C

更新日期：1992-12-21 00:00:00

abstract：:Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and d...

journal_title：FEBS letters

authors： Brown II,Fadeyev SI,Kirik II,Severina II,Skulachev VP

更新日期：1990-09-17 00:00:00

abstract：:In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...

journal_title：FEBS letters

authors： Prado A,Muga A,Castresana J,Goñi FM,Arrondo JL

更新日期：1990-09-03 00:00:00

abstract：:Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and p...

journal_title：FEBS letters

authors： Iwamoto Y,Tsubaki M,Hiwatashi A,Ichikawa Y

更新日期：1988-06-06 00:00:00

abstract：:Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...

journal_title：FEBS letters

authors： Barlati S,Paracini F,Bellotti D,De Petro G

更新日期：1991-04-09 00:00:00

abstract：:Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...

journal_title：FEBS letters

authors： Zhen WP,Jeppesen C,Nielsen PE

更新日期：1988-02-29 00:00:00