Abstract:
:In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been independently monitored, and correlations among the various parameters have been observed. The thermal denaturation of sarcoplasmic reticulum proteins in the presence of sodium dodecylsulphate has also been characterized spectroscopically.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Prado A,Muga A,Castresana J,Goñi FM,Arrondo JLdoi
10.1016/0014-5793(90)81185-qsubject
Has Abstractpub_date
1990-09-03 00:00:00pages
324-7issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81185-Qjournal_volume
269pub_type
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