Abstract:
:The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quantitation is achieved by (31)P-NMR determination of the composition of mixed micelles in the range of bilayer-micelle coexistence. We find that the solubilized fraction of bilayers consisting of binary mixtures of PC and SM is rich in SM, as expected from previous data on solubilization of pure PC and pure SM liposomes. In contrast, after partial solubilization of ternary mixtures of PC, SM and Chol, the solubilized fraction becomes SM-poor, as observed in the partial solubilization of biomembranes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ahyayauch H,Collado MI,Goñi FM,Lichtenberg Ddoi
10.1016/j.febslet.2009.07.046subject
Has Abstractpub_date
2009-09-03 00:00:00pages
2859-64issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00596-1journal_volume
583pub_type
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