Abstract:
:A site-selective cAMP analog, 8-chloroadenosine 3',5'-cyclic monophosphate (8-Cl-cAMP), was demonstrated to be a potent inhibitor of both the monolayer and soft agar growth of normal rat kidney (NRK) fibroblasts that had been transformed with the v-Ki-ras oncogene or treated with transforming growth factor alpha (TGF alpha). The growth inhibition was dose dependent and reversible and was accompanied by reversion of the transformed phenotype, suppression of TGF alpha production, and a decrease in p21 ras protein levels. These effects of 8-Cl-cAMP were linked to the cAMP analog's selective modulation of the type I and type II cAMP-dependent protein kinase regulatory subunits, RI and RII, present in Ki-ras-transformed and TGF alpha-treated NRK cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tortora G,Ciardiello F,Ally S,Clair T,Salomon DS,Cho-Chung YSdoi
10.1016/0014-5793(89)80502-2subject
Has Abstractpub_date
1989-01-02 00:00:00pages
363-7issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80502-2journal_volume
242pub_type
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