Isolation and characterization of neuropeptide Y from porcine intestine.

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:The essential role of Factor VIII:C (FVIII:C, anti-hemophilia factor A) as a cofactor for Factor IXa-dependent activation of Factor X has been established. In this paper, we describe that capillary endothelial cells from bovine adrenal medulla express active FVIII:C gene. Accumulation of FVIII:C in conditioned media f...

journal_title：FEBS letters

authors： Banerjee DK,Tavárez JJ,Oliveira CM

更新日期：1992-07-13 00:00:00

abstract：:Structure/function relationships in troponin C are studied with vertebrate fast-twitch fibers by exchanging the skeletal troponin C with two bacterially synthesized recombinant proteins designed by site-directed mutagenesis of cardiac troponin C. One mutant (CBM1) contained an additional active site, by deleting Val-2...

journal_title：FEBS letters

authors： Gulati J,Babu A,Putkey JA

更新日期：1989-05-08 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:In photosystem II (PSII) the probability that energy absorbed by core antenna chlorophyll (Chl) is transferred to the reaction center (RC) is extremely high. Although close proximity between antenna Chl ensures a high transfer efficiency, relative pigment orientation can fractionally modify it. This level of refinemen...

journal_title：FEBS letters

authors： Vasil'ev S,Shen JR,Kamiya N,Bruce D

更新日期：2004-03-12 00:00:00

abstract：:The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

journal_title：FEBS letters

authors： Betters JL,Yu L

更新日期：2010-07-02 00:00:00

abstract：:Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

journal_title：FEBS letters

authors： Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

更新日期：2016-02-01 00:00:00

abstract：:The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...

journal_title：FEBS letters

authors： Ling KK,Siow NL,Choi RC,Tsim KW

更新日期：2005-04-25 00:00:00

abstract：:Abstract Hyperhomocysteinemia has recently been identified as an important risk factor for Alzheimer's disease (AD). One of the potential mechanisms underlying harmful effects of homocysteine (Hcy) is site-specific acylation of proteins at lysine residues by homocysteine thiolactone (HCTL). The accumulation of amyloid...

journal_title：FEBS letters

authors： Khodadadi S,Riazi GH,Ahmadian S,Hoveizi E,Karima O,Aryapour H

更新日期：2012-01-20 00:00:00

abstract：:Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...

journal_title：FEBS letters

authors： Wenthold RJ,Hunter C,Wada K,Dechesne CJ

更新日期：1990-12-10 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

journal_title：FEBS letters

authors： Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

更新日期：2018-03-01 00:00:00

abstract：:Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...

journal_title：FEBS letters

authors： Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz G

更新日期：1995-01-30 00:00:00

abstract：:In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...

journal_title：FEBS letters

authors： Kunishima N,Amada F,Fukuyama K,Kawamoto M,Matsunaga T,Matsubara H

更新日期：1996-01-15 00:00:00

abstract：:The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression p...

journal_title：FEBS letters

authors： Paukert M,Hidayat S,Gründer S

更新日期：2002-02-27 00:00:00

abstract：:We present here the purification and the analysis of the structural and functional properties of distinctin, a 5.4 kDa heterodimeric peptide with antimicrobial activity from the tree-frog Phyllomedusa distincta. This peptide was isolated from the crude extract of skin granular glands by different chromatographic steps...

journal_title：FEBS letters

authors： Batista CV,Scaloni A,Rigden DJ,Silva LR,Rodrigues Romero A,Dukor R,Sebben A,Talamo F,Bloch C

更新日期：2001-04-06 00:00:00

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...

journal_title：FEBS letters

authors： Roth B,Schwendimann B,Hughes GJ,Tzartos SJ,Barkas T

更新日期：1987-08-31 00:00:00

abstract：:Nuclear factor of activated T cells (NFATc4) has been implicated as a critical regulator of the cardiac development and hypertrophy. However, the mechanisms for regulating NFATc4 stability and transactivation remain unclear. We showed that NFATc4 protein was predominantly ubiquitinated through the formation of Lysine ...

journal_title：FEBS letters

authors： Fan Y,Xie P,Zhang T,Zhang H,Gu D,She M,Li H

更新日期：2008-12-10 00:00:00

abstract：:Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...

journal_title：FEBS letters

authors： Choi HJ,Park JH,Park JH,Lee KB,Oh SM

更新日期：2015-12-21 00:00:00

abstract：:Activation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60-80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this si...

journal_title：FEBS letters

authors： Murphy PM,Gallin EK,Tiffany HL,Malech HL

更新日期：1990-02-26 00:00:00

abstract：:Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is known to act as a lipid phosphatase hydrolyzing phosphatidylinositol (PI)(3,4,5)P(3) to PI(4,5)P(2). Since the PI3-kinase product, PI(3,4,5)P(3), is an important second messenger leading to the metabolic action of insulin, PTEN functions as a potent neg...

journal_title：FEBS letters

authors： Ishihara H,Sasaoka T,Kagawa S,Murakami S,Fukui K,Kawagishi Y,Yamazaki K,Sato A,Iwata M,Urakaze M,Ishiki M,Wada T,Yaguchi S,Tsuneki H,Kimura I,Kobayashi M

更新日期：2003-11-20 00:00:00

abstract：:Using model spontaneously reverting cell lines, c-jun, junB, junD and c-fos oncogene expression was investigated. c-jun, but not junB, junD or c-fos, was overexpressed in highly tumorigenic clones. The reversion of cells to the non-tumorigenic phenotype resulted in a dramatic decrease in c-jun expression. CAT assays r...

journal_title：FEBS letters

authors： Lavrovsky Y,Yefremov Y,Lavrovsky V

更新日期：1994-12-19 00:00:00

abstract：:Epigenetic silencing mechanisms play an important role in chemoresistance of human cancer. Here we report the upregulated expression of metastasis-associated protein 1 (MTA1), a component of the nucleosome remodeling deacetylation (NuRD) complex, in chemoresistant prostate cancer (PCa). MTA1 knockdown in PC-3 cells in...

journal_title：FEBS letters

authors： Yu L,Su YS,Zhao J,Wang H,Li W

更新日期：2013-08-19 00:00:00

abstract：:The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...

journal_title：FEBS letters

authors： Aktories K,Schultz G,Jakobs KH

更新日期：1983-05-30 00:00:00

abstract：:The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent ...

journal_title：FEBS letters

authors： van der Wilk F,Huisman MJ,Cornelissen BJ,Huttinga H,Goldbach R

更新日期：1989-03-13 00:00:00

abstract：:We focused on the functional involvement of transforming growth factor-beta-activated kinase 1 (TAK1) in transcriptional regulation of interleukin-2 (IL-2) in T cells. Costimulation of Jurkat cells with 12-O-tetradecanoylphorbol-13-acetate and A23187 leads to a rapid phosphorylation of TAK1 and TAK1-binding protein 1 ...

journal_title：FEBS letters

authors： Sakurai H,Singhirunnusorn P,Shimotabira E,Chino A,Suzuki S,Koizumi K,Saiki I

更新日期：2005-12-05 00:00:00

abstract：:Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperatur...

journal_title：FEBS letters

authors： Thomas K,Rutter A,Suller M,Harwood J,Lloyd D

更新日期：1998-03-20 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor ...

journal_title：FEBS letters

authors： Fukuda T,Ochi H,Sunamura S,Haiden A,Bando W,Inose H,Okawa A,Asou Y,Takeda S

更新日期：2015-10-24 00:00:00