Oxygen induces fatty acid (n-6)-desaturation independently of temperature in Acanthamoeba castellanii.

abstract：:In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...

journal_title：FEBS letters

authors： Glitscher W,Rüppel H

更新日期：1991-05-06 00:00:00

abstract：:The aspA gene, encoding the aspartyl-tRNA synthetase (AspRS) from the hyperthermophilic archaeon Pyrococcus sp. KOD1, was expressed in Escherichia coli. The KOD1 AspRS, which was purified to homogeneity and was shown to be functional in dimeric form, aminoacylated tRNA from KOD1. The optimum temperature for this activ...

journal_title：FEBS letters

authors： Fujiwara S,Lee SG,Haruki M,Kanaya S,Takagi M,Imanaka T

更新日期：1996-09-23 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

journal_title：FEBS letters

authors： Huang J,Xu LG,Liu T,Zhai Z,Shu HB

更新日期：2006-02-06 00:00:00

abstract：:Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar appa...

journal_title：FEBS letters

authors： Boulton IC,Gorringe AR,Carr RJ,Gorinsky B,Joannou CL,Evans RW

更新日期：1997-09-08 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing ba...

journal_title：FEBS letters

authors： Arima H,Ibrahim HR,Kinoshita T,Kato A

更新日期：1997-09-22 00:00:00

abstract：:The gene of ecotin, an E. coli proteinase inhibitor, was cloned, and by site-directed mutagenesis the active site residue of the protein, Met84, was mutated to Lys, Arg and Leu. The recombinant wild-type and mutant inhibitors were overexpressed in E. coli, purified to homogeneity and their inhibitory effects on trypsi...

journal_title：FEBS letters

authors： Pál G,Sprengel G,Patthy A,Gráf L

更新日期：1994-03-28 00:00:00

abstract：:Previous electrophysiological experiments characterized the Sec61 complex, which provides the aqueous path for entry of newly-synthesized polypeptides into the mammalian endoplasmic reticulum, as a highly dynamic channel that, once activated by precursor proteins, fluctuates between main open states with mean conducta...

journal_title：FEBS letters

authors： Erdmann F,Jung M,Eyrisch S,Lang S,Helms V,Wagner R,Zimmermann R

更新日期：2009-07-21 00:00:00

abstract：:Protein kinases play a crucial role in the regulation of cellular processes. Most eukaryotes reserve about 2.5% of their genes for protein kinases. We analysed the genome of the single-celled ciliate Paramecium tetraurelia and identified 2606 kinases, about 6.6% of its genes, representing the largest kinome to date. A...

journal_title：FEBS letters

authors： Bemm F,Schwarz R,Förster F,Schultz J

更新日期：2009-11-19 00:00:00

abstract：:With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

journal_title：FEBS letters

authors： Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

更新日期：1998-07-24 00:00:00

abstract：:In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the me...

journal_title：FEBS letters

authors： Liu Z,Ng EK,Liang NC,Deng YF,Leung BC,Chen GG

更新日期：2005-02-28 00:00:00

abstract：:The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nu...

journal_title：FEBS letters

authors： Vogel MC,Papadopoulos T,Müller-Hermelink HK,Drahovsky D,Pfeifer GP

更新日期：1988-08-15 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:Although it has been shown that phosphorylations of p53 serine its residues are critical events for the regulation of their function, the specific biological effects of each of these phosphorylations, especially at serine 392, remain to be elucidated. Serine 392 has been proposed to play a role in the tetramerization ...

journal_title：FEBS letters

authors： Kim YY,Park BJ,Kim DJ,Kim WH,Kim S,Oh KS,Lim JY,Kim J,Park C,Park SI

更新日期：2004-08-13 00:00:00

abstract：:L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...

journal_title：FEBS letters

authors： Singer-Lahat D,Gershon E,Lotan I,Hullin R,Biel M,Flockerzi V,Hofmann F,Dascal N

更新日期：1992-07-20 00:00:00

abstract：:Incubation of isolated hepatocytes containing normal Ca2+ levels with angiotensin II, vasopressin or A23187 caused significant inhibition of the cAMP response to glucagon. Angiotensin II also inhibited cAMP accumulation induced by either glucagon or epinephrine in Ca2+-depleted hepatocytes. When submaximal doses of ho...

journal_title：FEBS letters

authors： Morgan NG,Exton JH,Blackmore PF

更新日期：1983-03-07 00:00:00

abstract：:The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

journal_title：FEBS letters

authors： Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

更新日期：1987-09-14 00:00:00

abstract：:1H-NMR spectroscopy has been applied to identify components in the urine of subjects with a deficiency of the enzyme 3-hydroxy-3-methylglutaryl-CoA lyase. One-dimensional spectra of samples from a pair of non-identical twins with this disorder were very similar and are probably diagnostic. The most intense signals wer...

journal_title：FEBS letters

authors： Iles RA,Jago JR,Williams SR,Chalmers RA

更新日期：1986-07-14 00:00:00

abstract：:The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), usin...

journal_title：FEBS letters

authors： Shi XL,Sun XY,Dalal NS

更新日期：1990-10-01 00:00:00

abstract：:An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

journal_title：FEBS letters

authors： Andralojc PJ,Harris DA

更新日期：1992-09-28 00:00:00

abstract：:Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

journal_title：FEBS letters

authors： Welker E,Narayan M,Volles MJ,Scheraga HA

更新日期：1999-11-05 00:00:00

abstract：:Potential antioxidant properties of therapeutically achievable concentrations of the protonated, active form of omeprazole (OM) were investigated in vitro at specific acidic pH values to mimic intragastric conditions in the clinical setting. We found that OM is a powerful scavenger of hypochlorous acid (HOCl) even at ...

journal_title：FEBS letters

authors： Lapenna D,de Gioia S,Ciofani G,Festi D,Cuccurullo F

更新日期：1996-03-11 00:00:00

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...

journal_title：FEBS letters

authors： Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GG

更新日期：1990-08-20 00:00:00

abstract：:The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...

journal_title：FEBS letters

authors： Toutant M,Bockaert J,Homburger V,Rouot B

更新日期：1987-09-28 00:00:00

abstract：:HGTD-P is a hypoxia-responsive pro-apoptotic protein that transmits hypoxic signals directly to mitochondria. When overexpressed, HGTD-P induces cell death via typical mitochondrial apoptotic cascades. However, much is unknown about post-transcriptional modification and signaling networks of HGTD-P in association with...

journal_title：FEBS letters

authors： Kim JY,Kim SM,Ko JH,Yim JH,Park JH,Park JH

更新日期：2006-05-29 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

journal_title：FEBS letters

authors： Law GJ,Ray KP,Wallis M

更新日期：1984-01-23 00:00:00

abstract：:The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential ...

journal_title：FEBS letters

authors： Menezes MC,Paes Leme AF,Melo RL,Silva CA,Della Casa M,Bruni FM,Lima C,Lopes-Ferreira M,Camargo AC,Fox JW,Serrano SM

更新日期：2008-11-26 00:00:00