Abstract:
:1H-NMR spectroscopy has been applied to identify components in the urine of subjects with a deficiency of the enzyme 3-hydroxy-3-methylglutaryl-CoA lyase. One-dimensional spectra of samples from a pair of non-identical twins with this disorder were very similar and are probably diagnostic. The most intense signals were from singlets. Complete assignment of these major components was made possible by the use of 2-dimensional chemical shift correlated spectroscopy since several long-range couplings were detected. 2-dimensional spectroscopic techniques may therefore be of value in the identification of singlets in multicomponent systems.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Iles RA,Jago JR,Williams SR,Chalmers RAdoi
10.1016/0014-5793(86)81434-xsubject
Has Abstractpub_date
1986-07-14 00:00:00pages
49-53issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)81434-Xjournal_volume
203pub_type
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