Abstract:
:We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [35S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial cells. However, the electrophoretic analysis and corresponding autoradiography of the glycosaminoglycans removed from the endothelial cell surface shows the presence of heparin-like compound which is not synthesized by the cells as it is unlabelled by [35S]sodium sulfate. We show here that a proteolytic treatment of the commonly used serum for cell culturing, reveals the presence of an heparin-like anticoagulant activity. These results suggest that the endothelial cells bind endogenous heparin-like molecules present in serum.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cavari S,Vannucchi Sdoi
10.1016/0014-5793(93)81469-gsubject
Has Abstractpub_date
1993-05-24 00:00:00pages
155-8issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)81469-Gjournal_volume
323pub_type
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