A plausible mechanism for large-scale chromosomal DNA amplification in streptomycetes.

abstract：:Two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) was used to assign the proton resonances of ferricytochrome C553 from Desulfovibrio vulgaris Hildenborough. The spin systems of 76 out of 79 amino acids were identified by J-correlation spectroscopy (COSY and HOHAHA) in H20 and D20 and correlated by nucl...

journal_title：FEBS letters

authors： Blanchard L,Blackledge MJ,Marion D,Guerlesquin F

更新日期：1996-07-01 00:00:00

abstract：:The kinetics of AMP-aminohydrolase, which under steady state conditions shows a typical sigmoid dependence of initial velocities versus substrate concentration, have been examined by rapid mixing methods. Using this technique it was observed that when substrate or substrate plus activator (K(+)) were mixed with enzyme...

journal_title：FEBS letters

authors： Suelter CH,Kovacs AL,Antonini E

更新日期：1968-11-01 00:00:00

abstract：:Impaired functioning of the proteasome pathway is one of the molecular mechanism underlying neurodegenerative changes in Alzheimer's disease. In this study, we report that dysfunction of the proteasome pathway in astroglial cells leads to decreased survival and dysregulation of chemokines by differential regulation of...

journal_title：FEBS letters

authors： Choi K,Lee J,Choi C

更新日期：2007-10-02 00:00:00

abstract：:The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

journal_title：FEBS letters

authors： Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

更新日期：1990-05-07 00:00:00

abstract：:Kaempferol is a dietary flavonoid that is thought to function as a selective estrogen receptor modulator. In this study, we established that kaempferol also functions as an inverse agonist for estrogen-related receptors alpha and gamma (ERRalpha and ERRgamma). We demonstrated that kaempferol binds to ERRalpha and ERRg...

journal_title：FEBS letters

authors： Wang J,Fang F,Huang Z,Wang Y,Wong C

更新日期：2009-02-18 00:00:00

abstract：:The chemotherapeutic activity of three synthetic antibacterial peptides was investigated. KLKLLLLLKLK-NH2 and its D-enantiomer showed significant chemotherapeutic activity in MRSA-infected mice, whereas KLKLLLKLK-NH2, which showed the highest antibacterial activity among them in vitro, was found to have almost no abil...

journal_title：FEBS letters

authors： Nakajima Y,Alvarez-Bravo J,Cho J,Homma K,Kanegasaki S,Natori S

更新日期：1997-09-22 00:00:00

abstract：:Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...

journal_title：FEBS letters

authors： Tachibanaki S,Imai H,Terakita A,Shichida Y

更新日期：1998-03-20 00:00:00

abstract：:Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1...

journal_title：FEBS letters

authors： Katsura H,Zikihara K,Okajima K,Yoshihara S,Tokutomi S

更新日期：2009-02-04 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...

journal_title：FEBS letters

authors： Okai H,Ikema R,Nakamura H,Kato M,Araki M,Mizuno A,Ikeda A,Renbaum P,Segel R,Funato K

更新日期：2020-08-01 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:The adiponectin paralog CORS-26 (collagenous repeat-containing sequence of 26kDa protein) is a member of the C1q/TNF-alpha molecular superfamily. CORS-26 is a secreted protein and baculovirus-produced CORS-26 released in the supernatant of insect cells forms stable trimers. Adiponectin exerts anti-inflammatory effects...

journal_title：FEBS letters

authors： Weigert J,Neumeier M,Schäffler A,Fleck M,Schölmerich J,Schütz C,Buechler C

更新日期：2005-10-24 00:00:00

abstract：:HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...

journal_title：FEBS letters

authors： Seong IS,Oh JY,Yoo SJ,Seol JH,Chung CH

更新日期：1999-07-30 00:00:00

abstract：:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks ...

journal_title：FEBS letters

authors： Loukin SH,Su Z,Kung C

更新日期：2009-02-18 00:00:00

abstract：:We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...

journal_title：FEBS letters

authors： Epand RM,Stafford A,Wang J,Epand RF

更新日期：1992-06-15 00:00:00

abstract：:A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome a...

journal_title：FEBS letters

authors： Brown L,Chen MX,Cohen PT

更新日期：1994-02-14 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:Partially reduced metabolites of molecular oxygen, superoxide (O2-) and hydrogen peroxide (H2O2), are detected in respiratory tract lining fluid, and it is assumed that these are key components of innate immunity. Whether these reactive oxygen species (ROS) are produced specifically by the respiratory epithelium in re...

journal_title：FEBS letters

authors： Harper RW,Xu C,Eiserich JP,Chen Y,Kao CY,Thai P,Setiadi H,Wu R

更新日期：2005-08-29 00:00:00

abstract：:It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the pr...

journal_title：FEBS letters

authors： Mizutani T,Maruyama N,Hitaka T,Sukenaga Y

更新日期：1989-04-24 00:00:00

abstract：:Adiponectin and resistin, two recently identified adipocyte-specific secretory factors, are able to modulate insulin actions in target tissues. To investigate their expression and hormonal regulation in brown adipocytes, we used the brown adipocyte cell line T37i, which, beside uncoupling protein expression, secretes ...

journal_title：FEBS letters

authors： Viengchareun S,Zennaro MC,Pascual-Le Tallec L,Lombes M

更新日期：2002-12-18 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00

abstract：:Activated B61.SF.1 and CTLL-2 T lymphocyte clones which are strictly dependent on interleukin-2 (IL-2) for growth were used to study the activation of Na+/K+-ATPase. 50% of [3H]thymidine maximal incorporation was obtained when the extracellular concentration of Na+ or K+ was reduced to 50 or 2 mM, respectively. 'Quies...

journal_title：FEBS letters

authors： Redondo JM,López Rivas A,Fresno M

更新日期：1986-10-06 00:00:00

abstract：:Connexin 43 (Cx43) hemichannels may form open channels in the plasma membrane when exposed to specific stimuli, e.g. reduced extracellular concentration of divalent cations, and allow passage of fluorescent molecules and presumably a range of smaller physiologically relevant molecules. However, the permeability profil...

journal_title：FEBS letters

authors： Hansen DB,Braunstein TH,Nielsen MS,MacAulay N

更新日期：2014-04-17 00:00:00

abstract：:Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

journal_title：FEBS letters

authors： He X,Chen X,Li B,Ji J,Chen S

更新日期：2017-12-01 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...

journal_title：FEBS letters

authors： Schnare MN,Greenwood SJ,Gray MW

更新日期：1995-03-27 00:00:00

abstract：:The Candida utilis QCR7 gene encoding subunit VII of the ubiquinol-cytochrome c oxidoreductase was isolated by functional complementation of the Saccharomyces cerevisiae subunit VII-null mutant. Several other subunit VII homologues as well as homologues for subunit VIII were identified by screening the GenBank databas...

journal_title：FEBS letters

authors： Boumans H,Berden JA,Grivell LA

更新日期：1995-07-10 00:00:00

abstract：:A 122 nt uridine-rich sequence (URE) in the Na(+)/glucose cotransporter (SGLT1) mRNA 3'-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK(1) cells stably expressing beta-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated ...

journal_title：FEBS letters

authors： Loflin P,Lever JE

更新日期：2001-03-16 00:00:00

abstract：:Islet-activating protein (IAP) suppressed Ca2+-induced histamine release and phospholipase A2 activation in Gpp(NH)p-loaded mast cells. Since the guanine nucleotide-binding protein involved in adenylate cyclase inhibition is known to lose its function upon being ADP-ribosylated by IAP, the nucleotide-binding protein i...

journal_title：FEBS letters

authors： Nakamura T,Ui M

更新日期：1984-08-06 00:00:00

abstract：:Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...

journal_title：FEBS letters

authors： Heindel U,Schmidt MF,Veit M

更新日期：2003-06-05 00:00:00

abstract：:To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...

journal_title：FEBS letters

authors： Sierant M,Leszczynska G,Sadowska K,Komar P,Radzikowska-Cieciura E,Sochacka E,Nawrot B

更新日期：2018-07-01 00:00:00