Abstract:
:HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with maltose binding protein (MBP). HslVU degraded both proteins upon incubation with ATP but not with its nonhydrolyzable analog, ATPgammaS, indicating that the degradation of SulA requires ATP hydrolysis. The pulse-chase experiment using an antibody raised against MBP-SulA revealed that the stability of SulA increased in hsl mutants and further increased in lon/hsl double mutants, indicating that SulA is an in vivo substrate of HslVU as well as of protease La (Lon). These results suggest that HslVU in addition to Lon plays an important role in regulation of cell division through degradation of SulA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Seong IS,Oh JY,Yoo SJ,Seol JH,Chung CHdoi
10.1016/s0014-5793(99)00935-7keywords:
subject
Has Abstractpub_date
1999-07-30 00:00:00pages
211-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00935-7journal_volume
456pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Binding of the thiazolyl peptide antibiotic thiostrepton to the GTPase domain of 23S rRNA involves a few crucial nucleotides, notably A1067 (E. coli). Small RNA transcripts were prepared corresponding to the GTPase domain of the plastid 23S rRNA and the two forms of cytosolic 28S rRNAs found in the human malaria paras...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00241-x
更新日期:1997-04-07 00:00:00
abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80911-4
更新日期:1984-10-15 00:00:00
abstract::Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80776-f
更新日期:1990-05-07 00:00:00
abstract::Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.038
更新日期:2007-10-30 00:00:00
abstract::The enzyme telomerase is necessary for the synthesis and maintenance of telomere length. The catalytic subunit, telomerase reverse transcriptase (TERT), is regulated by interaction with the 90 kDa heat shock protein (HSP90) and by Akt-dependent phosphorylation. Here, we demonstrate that HSP90 and Akt physically intera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00058-9
更新日期:2003-02-11 00:00:00
abstract::Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81668-0
更新日期:1989-12-04 00:00:00
abstract::Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01495-0
更新日期:1997-01-27 00:00:00
abstract::The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03346-x
更新日期:2002-10-23 00:00:00
abstract::H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80258-2
更新日期:1987-07-27 00:00:00
abstract::The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00974-0
更新日期:1998-09-04 00:00:00
abstract::A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81053-1
更新日期:1989-09-11 00:00:00
abstract::The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00678-x
更新日期:1999-06-11 00:00:00
abstract::Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.006
更新日期:2006-03-06 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.011
更新日期:2010-07-02 00:00:00
abstract::Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00177-4
更新日期:1997-03-24 00:00:00
abstract::cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01183-1
更新日期:1998-10-09 00:00:00
abstract::Amylin, a 37 amino acid C-terminal amidated peptide is an integral part of secretory granules of pancreatic beta-cells. Utilizing a specific radioimmunoassay system we demonstrate in the present study a cosecretion of amylin and insulin from the isolated rat pancreas. The secretion pattern of both peptides during gluc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80210-a
更新日期:1990-03-26 00:00:00
abstract::CREB binding protein (CBP) is an acetyltransferase that plays an important role in many biological processes. Here, we show that Akt phosphorylates CBP at threonine 1871 and suppresses its acetyltransferase activity by impeding the binding of CBP to histone H3, which results in a decrease in lysine K18 acetylation and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.02.023
更新日期:2013-04-02 00:00:00
abstract::Human seminal fluid contains a number of tripeptide amides with similar structures to thyrotropin releasing hormone (TRH), two of which have been identified as pGlu-Glu-Pro amide and pGlu-Phe-Pro amide. To determine whether these peptides originate in the same tissues and have the same molecular origin, TRH-immunoreac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01468-3
更新日期:1996-01-22 00:00:00
abstract::Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.017
更新日期:2014-05-02 00:00:00
abstract::In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.058
更新日期:2006-12-22 00:00:00
abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.034
更新日期:2007-05-15 00:00:00
abstract::Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03171-x
更新日期:2002-09-11 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::The dynamics of changes in membrane potential, cytosolic free Ca2+, [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80972-2
更新日期:1984-11-05 00:00:00
abstract::Promoter activity of protein kinase C (PKC) gamma gene was analysed by chloramphenicol acetyltransferase (CAT) assay using extracts from the cells transfected with various fusion constructs containing the 5'-flanking region of the mouse PKC gamma gene and CAT gene. Transient expression experiments in PC12 cells reveal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00665-v
更新日期:1995-07-17 00:00:00
abstract::The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibito...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80975-9
更新日期:1991-07-29 00:00:00
abstract::Amino acid sequences coded for by 13 different genes of mammalian mitochondrial DNA (mtDNA) including 8 unassigned open reading frames (URFs) were compared in pairs. It was found that significant homologies exist among the amino acid sequences of the three URFs (URF2, URF4 and URF5) with a probability of occurrence of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80847-4
更新日期:1985-09-09 00:00:00
abstract::HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.003
更新日期:2005-06-20 00:00:00
abstract::The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81277-f
更新日期:1991-10-21 00:00:00