Abstract:
:Promoter activity of protein kinase C (PKC) gamma gene was analysed by chloramphenicol acetyltransferase (CAT) assay using extracts from the cells transfected with various fusion constructs containing the 5'-flanking region of the mouse PKC gamma gene and CAT gene. Transient expression experiments in PC12 cells revealed that the upstream region of 87 bp from the transcriptional initiation site was sufficient for promoter activity. The region containing nucleotides 87 upstream from the transcriptional initiation site was shown to silence CAT activity in Balb/c3T3 cells, in which mRNA of PKC gamma was not detected, suggesting that this region might contain a transcriptional regulatory element for the cell type-specific expression of the PKC gamma gene.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Takanaga H,Mukai H,Shimakawa M,Konishi H,Kikkawa U,Koizumi T,Ono Ydoi
10.1016/0014-5793(95)00665-vsubject
Has Abstractpub_date
1995-07-17 00:00:00pages
276-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)00665-Vjournal_volume
368pub_type
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